ArticleEMBO reports2005
Crystal structure of a polyphosphate kinase and its implications for polyphosphate synthesis.
Article in EMBO reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 114 citations in OpenAlex.
- Probing metazoan polyphosphate biology usingeLife · 2026Article
- Genomic factors contributing to the resilience of Salmonella enterica on ready-to-eat muskmelon.Food microbiology · 2026Article
- Polyphosphate Polymerase-A Key Enzyme for the Phosphorus Economy of the Microalgal Cell and the Sustainable Usage of This Nutrient.Plants (Basel, Switzerland) · 2025Review
- Polyphosphate from Lactic Acid Bacteria: A Functional Molecule for Food and Health Applications.Foods (Basel, Switzerland) · 2025Review
- Light and polyphosphate kinase 2 cooperatively regulate the production of zero-valent sulfur in a deep-sea bacterium.mSystems · 2025Article
- Distribution of Polyphosphate Kinase 2 Genes in Bacteria Underscores a Dynamic Evolutionary History.Proteins · 2025Article
- A fusion protein of polyphosphate kinase 1 (PPK1) and a Nudix hydrolase is involved in inorganic polyphosphate accumulation in the unicellular red alga Cyanidioschyzon merolae.Plant molecular biology · 2024Article
- In vitro and in vivo activities of scutellarein, a novel polyphosphate kinase 1 inhibitor against Acinetobacter baumannii infection.Microbial cell factories · 2024Article
- C-terminal Poly-histidine Tags Alter Escherichia coli Polyphosphate Kinase Activity and Susceptibility to Inhibition.Journal of molecular biology · 2024Article
- An Update on Polyphosphate In Vivo Activities.Biomolecules · 2024Review
- The Protein Scaffolding Functions of Polyphosphate.Journal of molecular biology · 2024Review
- Polyphosphate-kinase-1 dependent polyphosphate hyperaccumulation for acclimation to nutrient loss in the cyanobacterium,Frontiers in plant science · 2024Article
- Assessing the influence of diverse phosphorus sources on bacterial communities and the abundance of phosphorus cycle genes in acidic paddy soils.Frontiers in microbiology · 2024Article
- Ddp1 Cooperates with Ppx1 to Counter a Stress Response Initiated by Nonvacuolar Polyphosphate.mBio · 2022Article
- Polyphosphate Kinase 2 (PPK2) Enzymes: Structure, Function, and Roles in Bacterial Physiology and Virulence.International journal of molecular sciences · 2022Review
- Make or break: the thermodynamic equilibrium of polyphosphate kinase-catalysed reactions.Beilstein journal of organic chemistry · 2022Article
- Tackling the Threat of Cancer Due to Pathobionts Producing Colibactin: Is Mesalamine the Magic Bullet?Toxins · 2021Review
- Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Did Cyclic Metaphosphates Have a Role in the Origin of Life?Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life · 2021Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Polyphosphate (polyP), a linear polymer of hundreds of orthophosphate residues, exists in all tested cells in nature, from pathogenic bacteria to mammals. In bacteria, polyP has a crucial role in stress responses and stationary-phase survival. Polyphosphate kinase (PPK) is the principal enzyme that catalyses the synthesis of polyP in bacteria. It has been shown that PPK is required for bacterial motility, biofilm formation and the production of virulence factors. PPK inhibitors may thus provide a unique therapeutic opportunity against antibiotic-resistant pathogens. Here, we report crystal structures of full-length Escherichia coli PPK and its complex with AMPPNP (beta-gamma-imidoadenosine 5-phosphate). PPK forms an interlocked dimer, with each 80 kDa monomer containing four structural domains. The PPK active site is located in a tunnel, which contains a unique ATP-binding pocket and may accommodate the translocation of synthesized polyP. The PPK structure has laid the foundation for understanding the initiation of polyP synthesis by PPK.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.