ArticleEukaryotic cell2005
Unique mitochondrial genome structure in diplonemids, the sister group of kinetoplastids.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
40 citing papers in PubMed, 98 citations in OpenAlex.
- Preparation of Diplonemid Samples for Microscopy.Methods in molecular biology (Clifton, N.J.) · 2026Article
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- Miniature RNAs are embedded in an exceptionally protein-rich mitoribosome via an elaborate assembly pathway.Nucleic acids research · 2023Article
- Origin of minicircular mitochondrial genomes in red algae.Nature communications · 2023Article
- Evolutionary History of Mitochondrial Genomes in Discoba, Including the Extreme Halophile Pleurostomum flabellatum (Heterolobosea).Genome biology and evolution · 2021Article
- Gene fragmentation and RNA editing without borders: eccentric mitochondrial genomes of diplonemids.Nucleic acids research · 2020Article
- Article
- Respiratory chain Complex I of unparalleled divergence in diplonemids.The Journal of biological chemistry · 2018Article
- Life Cycle, Ultrastructure, and Phylogeny of New Diplonemids and Their Endosymbiotic Bacteria.mBio · 2018Article
- Pervasive, Genome-Wide Transcription in the Organelle Genomes of Diverse Plastid-Bearing Protists.G3 (Bethesda, Md.) · 2017Article
- Keeping it complicated: Mitochondrial genome plasticity across diplonemids.Scientific reports · 2017Article
- Mitochondrial Genome Evolution and a Novel RNA Editing System in Deep-Branching Heteroloboseids.Genome biology and evolution · 2017Article
- Post-transcriptional mending of gene sequences: Looking under the hood of mitochondrial gene expression in diplonemids.RNA biology · 2016Review
- The unconventional kinetoplastid kinetochore: from discovery toward functional understanding.Biochemical Society transactions · 2016Review
- Hyper-eccentric structural genes in the mitochondrial genome of the algal parasite Hemistasia phaeocysticola.Genome biology and evolution · 2016Article
- Nothing in Evolution Makes Sense Except in the Light of Genomics: Read-Write Genome Evolution as an Active Biological Process.Biology · 2016Review
- From simple to supercomplex: mitochondrial genomes of euglenozoan protists.F1000Research · 2016Review
- Unexpectedly Streamlined Mitochondrial Genome of the Euglenozoan Euglena gracilis.Genome biology and evolution · 2015Article
- The massive mitochondrial genome of the angiosperm Silene noctiflora is evolving by gain or loss of entire chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- An improved method with a wider applicability to isolate plant mitochondria for mtDNA extraction.Plant methods · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetoplastid flagellates are characterized by uniquely massed mitochondrial DNAs (mtDNAs), the kinetoplasts. Kinetoplastids of the trypanosomatid group possess two types of mtDNA molecules: maxicircles bearing protein and mitoribosomal genes and minicircles specifying guide RNAs, which mediate uridine insertion/deletion RNA editing. These circles are interlocked with one another to form dense networks. Whether these peculiar mtDNA features are restricted to kinetoplastids or prevail throughout Euglenozoa (euglenids, diplonemids, and kinetoplastids) is unknown. Here, we describe the mitochondrial genome and the mitochondrial ultrastructure of Diplonema papillatum, a member of the diplonemid flagellates, the sister group of kinetoplastids. Fluorescence and electron microscopy show a single mitochondrion per cell with an ultrastructure atypical for Euglenozoa. In addition, DNA is evenly distributed throughout the organelle rather than compacted. Molecular and electron microscopy studies distinguish numerous 6- and 7-kbp-sized mitochondrial chromosomes of monomeric circular topology and relaxed conformation in vivo. Remarkably, the cox1 gene (and probably other mitochondrial genes) is fragmented, with separate gene pieces encoded on different chromosomes. Generation of the contiguous cox1 mRNA requires trans-splicing, the precise mechanism of which remains to be determined. Taken together, the mitochondrial gene/genome structure of Diplonema is not only different from that of kinetoplastids but unique among eukaryotes as a whole.
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