ArticleEukaryotic cell2005
A Rac homolog functions downstream of Ras1 to control hyphal differentiation and high-temperature growth in the pathogenic fungus Cryptococcus neoformans.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 66 citations in OpenAlex.
- Roles of Different Signaling Pathways inJournal of fungi (Basel, Switzerland) · 2024Review
- Review
- Environmental Factors That Contribute to the Maintenance ofMicroorganisms · 2020Review
- SH3-class Ras guanine nucleotide exchange factors are essential for Aspergillus fumigatus invasive growth.Cellular microbiology · 2019Article
- Article
- Ras signalling in pathogenic yeasts.Microbial cell (Graz, Austria) · 2017Review
- The role of Aspartyl aminopeptidase (Ape4) in Cryptococcus neoformans virulence and authophagy.PloS one · 2017Article
- RacA-Mediated ROS Signaling Is Required for Polarized Cell Differentiation in Conidiogenesis of Aspergillus fumigatus.PloS one · 2016Article
- Orchestration of Morphogenesis in Filamentous Fungi: Conserved Roles for Ras Signaling Networks.Fungal biology reviews · 2015Article
- New technology and resources for cryptococcal research.Fungal genetics and biology : FG & B · 2015Review
- Fungal morphogenesis.Cold Spring Harbor perspectives in medicine · 2014Review
- Molecular mechanisms of hypoxic responses via unique roles of Ras1, Cdc24 and Ptp3 in a human fungal pathogen Cryptococcus neoformans.PLoS genetics · 2014Article
- Small-GTPase-associated signaling by the guanine nucleotide exchange factors CpDock180 and CpCdc24, the GTPase effector CpSte20, and the scaffold protein CpBem1 in Claviceps purpurea.Eukaryotic cell · 2014Article
- Cryptococcus neoformans: historical curiosity to modern pathogen.Yeast (Chichester, England) · 2014Review
- Stress signaling pathways for the pathogenicity of Cryptococcus.Eukaryotic cell · 2013Review
- Restricted substrate specificity for the geranylgeranyltransferase-I enzyme in Cryptococcus neoformans: implications for virulence.Eukaryotic cell · 2013Article
- Two Rac paralogs regulate polarized growth in the human fungal pathogen Cryptococcus neoformans.Fungal genetics and biology : FG & B · 2013Article
- Ras1 acts through duplicated Cdc42 and Rac proteins to regulate morphogenesis and pathogenesis in the human fungal pathogen Cryptococcus neoformans.PLoS genetics · 2013Article
- Pleiotropic roles of the Msi1-like protein Msl1 in Cryptococcus neoformans.Eukaryotic cell · 2012Article
- Effect of ambient temperature on the clinical manifestations of experimental diffuse cutaneous leishmaniasis in a rodent model.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2012Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The Cryptococcus neoformans Ras1 protein serves as a central regulator for several signaling pathways. Ras1 controls the induction of the mating pheromone response cascade as well as a distinct signaling pathway that allows this pathogenic fungus to grow at human physiological temperature. To characterize elements of the Ras1-dependent high-temperature growth pathway, we performed a multicopy suppressor screen, identifying genes whose overexpression allows the ras1 mutant to grow at 37 degrees C. Using this genetic technique, we identified a C. neoformans gene encoding a Rac homolog that suppresses multiple ras1 mutant phenotypes. Deletion of the RAC1 gene does not affect high-temperature growth. However, a rac1 mutant strain demonstrates a profound defect in haploid filamentation as well as attenuated mating. In a yeast two-hybrid assay, Rac1 physically interacts with the PAK kinase Ste20, which similarly regulates hyphal formation in this fungus. Similar to Rac1, overexpression of the STE20alpha gene also restores high-temperature growth to the ras1 mutant. These results support a model in which the small G protein Rac1 acts downstream of Ras proteins and coordinately with Ste20 to control high-temperature growth and cellular differentiation in this human fungal pathogen.
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