ArticleNucleic acids research2005
A spontaneous tRNA suppressor of a mutation in the Chlamydomonas reinhardtii nuclear MCD1 gene required for stability of the chloroplast petD mRNA.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 33 citations in OpenAlex.
- Towards green biomanufacturing of high-value recombinant proteins using promising cell factory: Chlamydomonas reinhardtii chloroplast.Bioresources and bioprocessing · 2022Review
- A novel mistranslating tRNA model in Drosophila melanogaster has diverse, sexually dimorphic effects.G3 (Bethesda, Md.) · 2022Article
- Pas de Trois: An Overview of Penta-, Tetra-, and Octo-Tricopeptide Repeat Proteins FromFrontiers in plant science · 2021Review
- The OPR Protein MTHI1 Controls the Expression of Two Different Subunits of ATP Synthase CFo inThe Plant cell · 2020Article
- Small RNA profiling in Chlamydomonas: insights into chloroplast RNA metabolism.Nucleic acids research · 2017Article
- High-throughput droplet microfluidics screening platform for selecting fast-growing and high lipid-producing microalgae from a mutant library.Plant direct · 2017Article
- The Octatricopeptide Repeat Protein Raa8 Is Required for Chloroplast trans Splicing.Eukaryotic cell · 2015Article
- Flux balance analysis reveals acetate metabolism modulates cyclic electron flow and alternative glycolytic pathways in Chlamydomonas reinhardtii.Frontiers in plant science · 2015Article
- RAP, the sole octotricopeptide repeat protein in Arabidopsis, is required for chloroplast 16S rRNA maturation.The Plant cell · 2014Article
- The pentatricopeptide repeat MTSF1 protein stabilizes the nad4 mRNA in Arabidopsis mitochondria.Nucleic acids research · 2013Article
- Function of chloroplast RNA-binding proteins.Cellular and molecular life sciences : CMLS · 2011Review
- Mechanism of RNA stabilization and translational activation by a pentatricopeptide repeat protein.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Site-specific binding of a PPR protein defines and stabilizes 5' and 3' mRNA termini in chloroplasts.The EMBO journal · 2009Article
- Improving gene-finding in Chlamydomonas reinhardtii:GreenGenie2.BMC genomics · 2009Article
- Molecular identification and function of cis- and trans-acting determinants for petA transcript stability in Chlamydomonas reinhardtii chloroplasts.Molecular and cellular biology · 2008Article
- Evidence for regulatory function of nucleus-encoded factors on mRNA stabilization and translation in the chloroplast.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Regulatory sequences of orthologous petD chloroplast mRNAs are highly specific among Chlamydomonas species.Plant molecular biology · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Numerous nuclear gene products are required for the correct expression of organellar genes. One such gene in the green alga Chlamydomonas reinhardtii is MCD1, whose product is required for stability of the chloroplast-encoded petD mRNA. In mcd1 mutants, which are non-photosynthetic, petD mRNA is degraded by a 5'-3' exonuclease activity, resulting in a failure to synthesize its product, subunit IV of the cytochrome b (6)/f complex. Here, we report the sequence of the wild-type MCD1 gene, which encodes a large and novel putative protein. Analysis of three mutant alleles showed that two harbored large deletions, but that one allele, mcd1-2, had a single base change resulting in a nonsense codon near the N-terminus. This same mutant allele can be suppressed by a second-site mutation in the nuclear MCD2 gene, whereas mcd2-1 cannot suppress the deletion in mcd1-1 (Esposito,D. Higgs,D.C. Drager,R.G. Stern, D.B. and Girard-Bascou,J. (2001) Curr. Genet., 39, 40-48). We report the cloning of mcd2-1, and show that the mutation lies in a tRNA(Ser)(CGA), which has been modified to translate the nonsense codon in mcd1-2. We discuss how the existence of a large tRNA(Ser) gene family may permit this suppression without pleiotropic consequences.
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