ArticleNucleic acids research2005
A real-time semi-quantitative RT-PCR assay demonstrates that the pilE sequence dictates the frequency and characteristics of pilin antigenic variation in Neisseria gonorrhoeae.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 21 citations in OpenAlex.
- Article
- PacBio Amplicon Sequencing Method To Measure Pilin Antigenic Variation Frequencies of Neisseria gonorrhoeae.mSphere · 2019Article
- Analyzing Neisseria gonorrhoeae Pilin Antigenic Variation Using 454 Sequencing Technology.Journal of bacteriology · 2016Article
- Antigenic Variation in Bacterial Pathogens.Microbiology spectrum · 2016Article
- The use of high-throughput DNA sequencing in the investigation of antigenic variation: application to Neisseria species.PloS one · 2014Article
- Neisseria gonorrhoeae RecQ helicase HRDC domains are essential for efficient binding and unwinding of the pilE guanine quartet structure required for pilin antigenic variation.Journal of bacteriology · 2013Article
- Suggested role for G4 DNA in recombinational switching at the antigenic variation locus of the Lyme disease spirochete.PloS one · 2013Article
- Microbial antigenic variation mediated by homologous DNA recombination.FEMS microbiology reviews · 2012Review
- The Neisseria gonorrhoeae photolyase orthologue phrB is required for proper DNA supercoiling but does not function in photo-reactivation.Molecular microbiology · 2011Article
- Expression of the apoptosis-related proteins caspase-3 and NF-kappaB in the hippocampus of Tg2576 mice.Neuroscience bulletin · 2010Article
- Algorithmic assessment of vaccine-induced selective pressure and its implications on future vaccine candidates.Advances in bioinformatics · 2010Article
- Mismatch correction modulates mutation frequency and pilus phase and antigenic variation in Neisseria gonorrhoeae.Journal of bacteriology · 2010Article
- Pilin antigenic variation occurs independently of the RecBCD pathway in Neisseria gonorrhoeae.Journal of bacteriology · 2009Article
- Pilin gene variation in Neisseria gonorrhoeae: reassessing the old paradigms.FEMS microbiology reviews · 2009Review
- Conserved regions from Neisseria gonorrhoeae pilin are immunosilent and not immunosuppressive.Infection and immunity · 2007Article
- Loss of both Holliday junction processing pathways is synthetically lethal in the presence of gonococcal pilin antigenic variation.Molecular microbiology · 2006Article
- The obligate human pathogen, Neisseria gonorrhoeae, is polyploid.PLoS biology · 2006Article
- The frequency and rate of pilin antigenic variation in Neisseria gonorrhoeae.Molecular microbiology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
A semi-quantitative real-time RT-PCR assay was designed to measure gonococcal pilin antigenic variation (SQ-PCR Av assay). This assay employs 17 hybridization probe sets that quantitate subpopulations of pilin transcripts carrying different silent pilin copy sequences and one set that detects total pilE transcript levels. Mixtures of a DNA standard carrying the silent copy being detected and a clone encoding the starting pilE sequence, which is the majority pilE template, provided amplification curves that closely matched the experimental data and allowed an analysis of the contribution of different silent pilin copies to variation. The SQ-PCR Av assay was verified using DNA sequence analysis to demonstrate that this methodology allowed an accurate analysis of pilin variation. Both assays showed that with a specific starting pilE sequence, only a subset of the silent pilin copies recombine into pilE at a detectable level, and that this limited subset was reproducibly detected in replicate cultures. When an isogenic pilE sequence variant was examined using both assays, a new subset of silent copy sequences were detected recombining into pilE and the overall frequency of variation was increased. Thus, the parental pilE sequence influences the frequency of variation and the repertoire of pilin variants produced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.