ArticleNucleic acids research1992
Reduction of a conserved Cys is essential for Myb DNA-binding.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 129 citations in OpenAlex.
- The PavMYB.C2-UFGT module contributes to fruit coloration via modulating anthocyanin biosynthesis in sweet cherry.PLoS genetics · 2025Article
- The Role ofBiomedicines · 2024Review
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- Are Flavonoids Effective Antioxidants in Plants? Twenty Years of Our Investigation.Antioxidants (Basel, Switzerland) · 2020Review
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- Cysteine Dioxygenase 1 Mediates Erastin-Induced Ferroptosis in Human Gastric Cancer Cells.Neoplasia (New York, N.Y.) · 2017Article
- Genome-wide analysis of the R2R3-MYB transcription factor gene family in sweet orange (Citrus sinensis).Molecular biology reports · 2014Article
- FlbD, a Myb transcription factor of Aspergillus nidulans, is uniquely involved in both asexual and sexual differentiation.Eukaryotic cell · 2012Article
- A highly organized structure mediating nuclear localization of a Myb2 transcription factor in the protozoan parasite Trichomonas vaginalis.Eukaryotic cell · 2011Article
- Nuclear translocation of haeme oxygenase-1 is associated to prostate cancer.British journal of cancer · 2007Article
- Multifarious transcriptional regulation of adhesion protein gene ap65-1 by a novel Myb1 protein in the protozoan parasite Trichomonas vaginalis.Eukaryotic cell · 2006Article
- Regulation of FeLV-945 by c-Myb binding and CBP recruitment to the LTR.Virology journal · 2004Article
- Evolutionary dynamics of the DNA-binding domains in putative R2R3-MYB genes identified from rice subspecies indica and japonica genomes.Plant physiology · 2004Article
- Highly conserved features of DNA binding between two divergent members of the Myb family of transcription factors.Nucleic acids research · 2001Article
- c-Myb acetylation at the carboxyl-terminal conserved domain by transcriptional co-activator p300.Oncogene · 2000Article
- Myb-related Schizosaccharomyces pombe cdc5p is structurally and functionally conserved in eukaryotes.Molecular and cellular biology · 1998Article
- Oxidant-induced activation of nuclear factor-kappa B in cultured guinea pig gastric epithelial cells.Digestive diseases and sciences · 1997Article
- Transcription factors and aging.Molecular medicine (Cambridge, Mass.) · 1997Review
- AP-1 transcriptional activity is regulated by a direct association between thioredoxin and Ref-1.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Regulation of reactive-oxygen-species generation in fibroblasts by Rac1.The Biochemical journal · 1996Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The human c-Myb gene product is a regulator of transcription with intrinsic DNA-binding activity located in two of three aminoterminal repeats R2R3. Three purified recombinant c-Myb proteins, a 42 kD protein corresponding to the amino-terminal half (HM42), and two proteins representing R2R3 or R1R2R3 (HMR23 and HMR123) have been analyzed either as purified proteins or present in bacterial extracts in gelshift analyses using a high-affinity DNA oligonucleotide. The purified proteins are inactive in DNA-binding unless supplemented with a reducing agent such as dithiothreitol (DTT) in vitro. Alternatively a cellular nuclear extract (Nex) from HeLa cells strongly activates the binding. This effect is dose-dependent and sensitive to heat. The Nex does not lead to changes in the Myb-DNA mobility shift assay excluding a direct association of the complex with a cellular component. Site-directed mutagenesis of the aminoacid residue 130, a single conserved cysteine in HMR23 to serine almost completely abolishes DNA binding. Oxidation by diamide or alkylation by N-ethylmaleimide (NEM) of the Myb-proteins in vitro inhibit their interaction with DNA whereby the diamide effect is reversible by addition of excess of DTT. Nex prepared from COS cells transfected with c-myb leads to Myb-DNA interaction which is not responsive to DTT but sensitive to NEM and diamide. Our data indicate that the reduced cysteine of Myb is essential for its DNA-binding and that Myb function may be regulated by a reduction-oxidation mechanism.
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