ArticleAmerican journal of physiology. Renal physiology2005
Glucocorticoids acutely increase cell surface Na+/H+ exchanger-3 (NHE3) by activation of NHE3 exocytosis.
Article in American journal of physiology. Renal physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 93 citations in OpenAlex.
- The use of plasma aldosterone and urinary sodium to potassium ratio as translatable quantitative biomarkers of mineralocorticoid receptor antagonism.Journal of translational medicine · 2011Trial
- Chronic ACTH Infusion Alters the Diurnal Rhythm of Sodium Excretion, Inducing Nondipping Blood Pressure and Salt-Sensitivity in Male Mice.Hypertension (Dallas, Tex. : 1979) · 2025Article
- Mechanisms and physiological relevance of acid-base exchange in functional units of the kidney.PeerJ · 2024Review
- Review
- The Post-Translational Modification Networking in WNK-Centric Hypertension Regulation and Electrolyte Homeostasis.Biomedicines · 2022Review
- mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology · 2022Article
- The NaFrontiers in physiology · 2022Review
- The recycling regulation of sodium-hydrogen exchanger isoform 3(NHE3) in epithelial cells.Cell cycle (Georgetown, Tex.) · 2021Review
- New Insights into the Critical Importance of Intratubular NaCurrent hypertension reports · 2021Review
- Genetic and genomic evidence for an important role of the NaPhysiological genomics · 2019Review
- Recent Updates on the Proximal Tubule Renin-Angiotensin System in Angiotensin II-Dependent Hypertension.Current hypertension reports · 2016Review
- Tetracyclines increase lipid phosphate phosphatase expression on plasma membranes and turnover of plasma lysophosphatidate.Journal of lipid research · 2016Article
- Acid-Base Homeostasis.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Urea and Ammonia Metabolism and the Control of Renal Nitrogen Excretion.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
- Sorting nexin 27 regulates basal and stimulated brush border trafficking of NHE3.Molecular biology of the cell · 2015Article
- Mechanisms of Intestinal Serotonin Transporter (SERT) Upregulation by TGF-β1 Induced Non-Smad Pathways.PloS one · 2015Article
- Renal NHE expression and activity in neonatal NHE3- and NHE8-null mice.American journal of physiology. Renal physiology · 2015Article
- Effect of postnatal maternal protein intake on prenatal programming of hypertension.Reproductive sciences (Thousand Oaks, Calif.) · 2014Article
- Molecular mechanisms and regulation of urinary acidification.Comprehensive Physiology · 2014Review
- Myosin VI mediates the movement of NHE3 down the microvillus in intestinal epithelial cells.Journal of cell science · 2014Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Glucocorticoids have important effects on renal function, including the modulation of renal acidification by the major proximal tubular Na(+)/H(+) exchanger, NHE3. While the chronic effect of glucocorticoids is considered to be primarily at the transcriptional level, with increases in NHE3 mRNA and protein expression driving increased transport activity, the mechanisms by which glucocorticoids activate NHE3 in an acute setting have not been investigated. Previous studies have shown that a glucocorticoid-stimulated increase in NHE3 activity can occur before any detectable change in NHE3 mRNA. The present study examines the acute effects of glucocorticoids on NHE3 using opossum kidney (OKP) cells as a cell model. In OKP cells, total NHE3 protein abundance was not changed by 3 h of treatment with dexamethasone (10(-6) M). However, the biotin-accessible fraction representing NHE3 at the apical membrane as well as Na(+)/H(+) exchange activity measured fluorimetrically using the pH-sensitive dye BCECF-AM were significantly increased. These effects were not prevented by the protein synthesis inhibitor cycloheximide. NHE3 insertion (biotinylatable NHE3 after sulfo-NHS-acetate blockade) was stimulated by dexamethasone incubation, with or without cycloheximide. The rate of NHE3 endocytic retrieval, assessed either by the avidin protection assay (early endocytosis) or by the sodium 2-mercaptoethane sulfonate (MesNa) cleavage assay (early and late endocytosis), was not affected by dexamethasone. These findings suggest that trafficking plays a key role in the acute stimulation of NHE3 by glucocorticoids, with exocytosis being the major contributor to the glucocorticoid-induced rapid increase in cell surface NHE3 protein abundance and Na(+)/H(+) exchange activity.
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