ArticleNucleic acids research2005
Visualizing the dynamic behavior of poliovirus plus-strand RNA in living host cells.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 55 citations in OpenAlex.
- Review
- In vitro RNA release from a human rhinovirus monitored by means of a molecular beacon and chip electrophoresis.Analytical and bioanalytical chemistry · 2016Article
- Visual detection of Flavivirus RNA in living cells.Methods (San Diego, Calif.) · 2016Review
- The use of nanocrystal quantum dot as fluorophore reporters in molecular beacon-based assays.Nano convergence · 2016Review
- Article
- Imaging of mRNA-protein interactions in live cells using novel mCherry trimolecular fluorescence complementation systems.PloS one · 2013Article
- Review
- Application of live-cell RNA imaging techniques to the study of retroviral RNA trafficking.Viruses · 2012Review
- Apical transport of influenza A virus ribonucleoprotein requires Rab11-positive recycling endosome.PloS one · 2011Article
- Phosphatidylinositol 3-kinase-, actin-, and microtubule-dependent transport of Semliki Forest Virus replication complexes from the plasma membrane to modified lysosomes.Journal of virology · 2010Article
- Dynamics of coronavirus replication-transcription complexes.Journal of virology · 2010Article
- Imaging intracellular RNA distribution and dynamics in living cells.Nature methods · 2009Review
- Site of human rhinovirus RNA uncoating revealed by fluorescent in situ hybridization.Journal of virology · 2009Article
- Imaging and characterizing influenza A virus mRNA transport in living cells.Nucleic acids research · 2008Article
- Detection of hepatitis a virus by using a combined cell culture-molecular beacon assay.Applied and environmental microbiology · 2008Article
- Molecular beacons in biomedical detection and clinical diagnosis.International journal of clinical and experimental pathology · 2008Article
- Dynamics of filamentous viral RNPs prior to egress.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dynamic analysis of viral nucleic acids in host cells is important for understanding virus-host interaction. By labeling endogenous RNA with molecular beacon, we have realized the direct visualization of viral nucleic acids in living host cells and have studied the dynamic behavior of poliovirus plus-strand RNA. Poliovirus plus-strand RNA was observed to display different distribution patterns in living Vero cells at different post-infection time points. Real-time imaging suggested that the translocation of poliovirus plus-strand RNA is a characteristic rearrangement process requiring intact microtubule network of host cells. Confocal-FRAP measurements showed that 49.4 +/- 3.2% of the poliovirus plus-strand RNA molecules diffused freely (with a D-value of 9.6 +/- 1.6 x 10(-10) cm2/s) within their distribution region, while the remaining (50.5 +/- 2.9%) were almost immobile and moved very slowly only with change of the RNA distribution region. Under the electron microscope, it was found that virus-induced membrane rearrangement is microtubule-associated in poliovirus-infected Vero cells. These results reveal an entrapment and diffusion mechanism for the movement of poliovirus plus-strand RNA in living mammalian cells, and demonstrate that the mechanism is mainly associated with microtubules and virus-induced membrane structures.
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