ArticleNucleic acids research2005
Genome amplification of single sperm using multiple displacement amplification.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Conventional IVF is feasible in preimplantation genetic testing for aneuploidy.Journal of assisted reproduction and genetics · 2023Article
- Recent advances and application of whole genome amplification in molecular diagnosis and medicine.MedComm · 2022Review
- Genome sequencing and de novo assembly of the giant unicellular alga Acetabularia acetabulum using droplet MDA.Scientific reports · 2021Article
- Evaluation of single-cell genomics to address evolutionary questions using three SAGs of the choanoflagellate Monosiga brevicollis.Scientific reports · 2017Article
- Direct squencing from the minimal number of DNA molecules needed to fill a 454 picotiterplate.PloS one · 2014Article
- Parallel single cancer cell whole genome amplification using button-valve assisted mixing in nanoliter chambers.PloS one · 2014Article
- Sequencing of isolated sperm cells for direct haplotyping of a human genome.Genome research · 2013Article
- Characterization of whole genome amplified (WGA) DNA for use in genotyping assay development.BMC genomics · 2012Article
- Article
- Whole genome amplification in preimplantation genetic diagnosis.Journal of Zhejiang University. Science. B · 2011Review
- Forensic trace DNA: a review.Investigative genetics · 2010Article
- Evaluation of genome coverage and fidelity of multiple displacement amplification from single cells by SNP array.Molecular human reproduction · 2009Article
- Specific and complete human genome amplification with improved yield achieved by phi29 DNA polymerase and a novel primer at elevated temperature.BMC research notes · 2009Article
- A procedure for highly specific, sensitive, and unbiased whole-genome amplification.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids.Environmental microbiology · 2008Article
- Amplification of multiple genomic loci from single cells isolated by laser micro-dissection of tissues.BMC biotechnology · 2008Article
- Multiple strand displacement amplification of mitochondrial DNA from clinical samples.BMC medical genetics · 2008Article
- Use of multiple displacement amplification in the investigation of human papillomavirus physical status.Journal of clinical pathology · 2007Article
- Increasing amplification success of forensic DNA samples using multiple displacement amplification.Forensic science, medicine, and pathology · 2007Article
- Use of multiple displacement amplification to increase the detection and genotyping of trypanosoma species samples immobilized on FTA filters.The American journal of tropical medicine and hygiene · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sperm typing is an effective way to study recombination rate on a fine scale in regions of interest. There are two strategies for the amplification of single meiotic recombinants: repulsion-phase allele-specific PCR and whole genome amplification (WGA). The former can selectively amplify single recombinant molecules from a batch of sperm but is not scalable for high-throughput operation. Currently, primer extension pre-amplification is the only method used in WGA of single sperm, whereas it has limited capacity to produce high-coverage products enough for the analysis of local recombination rate in multiple large regions. Here, we applied for the first time a recently developed WGA method, multiple displacement amplification (MDA), to amplify single sperm DNA, and demonstrated its great potential for producing high-yield and high-coverage products. In a 50 mul reaction, 76 or 93% of loci can be amplified at least 2500- or 250-fold, respectively, from single sperm DNA, and second-round MDA can further offer >200-fold amplification. The MDA products are usable for a variety of genetic applications, including sequencing and microsatellite marker and single nucleotide polymorphism (SNP) analysis. The use of MDA in single sperm amplification may open a new era for studies on local recombination rates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.