ArticleMicrobiology (Reading, England)2005
Binding and transcriptional activation of non-flagellar genes by the Escherichia coli flagellar master regulator FlhD2C2.
Article in Microbiology (Reading, England), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 69 citations in OpenAlex.
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- DNA methylation affects gene expression but not global chromatin structure inJournal of bacteriology · 2025Article
- Motility-activating mutations upstream ofMicrobiology spectrum · 2024Article
- Mechanism ofInternational journal of molecular sciences · 2023Article
- Investigating the Prevalence of RNA-Binding Metabolic Enzymes inInternational journal of molecular sciences · 2023Article
- Genomic SELEX Reveals Pervasive Role of the Flagella Master Regulator FlhDC in Carbon Metabolism.International journal of molecular sciences · 2023Article
- Molecular structure of the intact bacterial flagellar basal body.Nature microbiology · 2021Article
- The transcription regulator and c-di-GMP phosphodiesterase PdeL represses motility inJournal of bacteriology · 2020Article
- Innovation and Application of the Type III Secretion System Inhibitors in Plant Pathogenic Bacteria.Microorganisms · 2020Review
- The LysR-Type Transcriptional Regulator CrgA Negatively Regulates the Flagellar Master RegulatorJournal of bacteriology · 2020Article
- Filtering input fluctuations in intensity and in time underlies stochastic transcriptional pulses without feedback.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Peptidoglycan Endopeptidase Spr of UropathogenicFrontiers in microbiology · 2020Article
- Article
- Driving the expression of the Salmonella enterica sv Typhimurium flagellum using flhDC from Escherichia coli results in key regulatory and cellular differences.Scientific reports · 2018Article
- The evolutionary impact of intragenic FliA promoters in proteobacteria.Molecular microbiology · 2018Article
- Using RegulonDB, the Escherichia coli K-12 Gene Regulatory Transcriptional Network Database.Current protocols in bioinformatics · 2018Article
- Transcriptomic Analysis of the Swarm Motility Phenotype of Salmonella enterica Serovar Typhimurium Mutant Defective in Periplasmic Glucan Synthesis.Current microbiology · 2017Article
- Global Gene-expression Analysis of the Response ofFrontiers in microbiology · 2017Article
- The fitness effects of a point mutation in Escherichia coli change with founding population density.Genetica · 2016Article
- Cross-talk between a regulatory small RNA, cyclic-di-GMP signalling and flagellar regulator FlhDC for virulence and bacterial behaviours.Environmental microbiology · 2015Article
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Authors and funding
3 authors at 2 institutions in 1 country.
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Abstract
The gene hierarchy directing biogenesis of peritrichous flagella on the surface of Escherichia coli and other enterobacteria is controlled by the heterotetrameric master transcriptional regulator FlhD(2)C(2). To assess the extent to which FlhD(2)C(2) directly activates promoters of a wider regulon, a computational screen of the E. coli genome was used to search for gene-proximal DNA sequences similar to the 42-44 bp inverted repeat FlhD(2)C(2) binding consensus. This identified the binding sequences upstream of all eight flagella class II operons, and also putative novel FlhD(2)C(2) binding sites in the promoter regions of 39 non-flagellar genes. Nine representative non-flagellar promoter regions were all bound in vitro by active reconstituted FlhD(2)C(2) over the K(D) range 38-356 nM, and of the nine corresponding chromosomal promoter-lacZ fusions, those of the four genes b1904, b2446, wzz(fepE) and gltI showed up to 50-fold dependence on FlhD(2)C(2) in vivo. In comparison, four representative flagella class II promoters bound FlhD(2)C(2) in the K(D) range 12-43 nM and were upregulated in vivo 30- to 990-fold. The FlhD(2)C(2)-binding sites of the four regulated non-flagellar genes overlap by 1 or 2 bp the predicted -35 motif of the FlhD(2)C(2)-activated sigma(70) promoters, as is the case with FlhD(2)C(2)-dependent class II flagellar promoters. The data indicate a wider FlhD(2)C(2) regulon, in which non-flagellar genes are bound and activated directly, albeit less strongly, by the same mechanism as that regulating the flagella gene hierarchy.
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