ArticleBMC molecular biology2005
Nuclear distribution and chromatin association of DNA polymerase alpha-primase is affected by TEV protease cleavage of Cdc23 (Mcm10) in fission yeast.
Article in BMC molecular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Role of Cdc23/Mcm10 in generating the ribonucleotide imprint at the mat1 locus in fission yeast.Nucleic acids research · 2019Article
- Review
- Novel roles of HP1a and Mcm10 in DNA replication, genome maintenance and photoreceptor cell differentiation.Nucleic acids research · 2017Article
- Recruitment of Mcm10 to Sites of Replication Initiation Requires Direct Binding to the Minichromosome Maintenance (MCM) Complex.The Journal of biological chemistry · 2016Article
- Mcm10 deficiency causes defective-replisome-induced mutagenesis and a dependency on error-free postreplicative repair.Cell cycle (Georgetown, Tex.) · 2014Article
- Drosophila Mcm10 is required for DNA replication and differentiation in the compound eye.PloS one · 2014Article
- Enigmatic roles of Mcm10 in DNA replication.Trends in biochemical sciences · 2013Review
- Article
- Direct role for proliferating cell nuclear antigen in substrate recognition by the E3 ubiquitin ligase CRL4Cdt2.The Journal of biological chemistry · 2012Article
- Structural biology of replication initiation factor Mcm10.Sub-cellular biochemistry · 2012Review
- A novel DNA nuclease is stimulated by association with the GINS complex.Nucleic acids research · 2011Article
- Cdt1 proteolysis is promoted by dual PIP degrons and is modulated by PCNA ubiquitylation.Nucleic acids research · 2011Article
- Drosophila Ctf4 is essential for efficient DNA replication and normal cell cycle progression.BMC molecular biology · 2011Article
- Solution NMR structure of the C-terminal DNA binding domain of Mcm10 reveals a conserved MCM motif.The Journal of biological chemistry · 2010Article
- Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system.PloS one · 2009Article
- Physical interactions between Mcm10, DNA, and DNA polymerase alpha.The Journal of biological chemistry · 2009Article
- Ancient diversification of eukaryotic MCM DNA replication proteins.BMC evolutionary biology · 2009Article
- Article
- GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.Molecular biology of the cell · 2009Article
- Structural basis for DNA binding by replication initiator Mcm10.Structure (London, England : 1993) · 2008Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCdc23/Mcm10 is required for the initiation and elongation steps of DNA replication but its biochemical function is unclear. Here, we probe its function using a novel approach in fission yeast, involving Cdc23 cleavage by the TEV protease.
resultsInsertion of a TEV protease cleavage site into Cdc23 allows in vivo removal of the C-terminal 170 aa of the protein by TEV protease induction, resulting in an S phase arrest. This C-terminal fragment of Cdc23 is not retained in the nucleus after cleavage, showing that it lacks a nuclear localization signal and ability to bind to chromatin. Using an in situ chromatin binding procedure we have determined how the S phase chromatin association of DNA polymerase alpha-primase and the GINS (Sld5-Psf1-Psf2-Psf3) complex is affected by Cdc23 inactivation. The chromatin binding and sub-nuclear distribution of DNA primase catalytic subunit (Spp1) is affected by Cdc23 cleavage and also by inactivation of Cdc23 using a degron allele, implying that DNA polymerase alpha-primase function is dependent on Cdc23. In contrast to the effect on Spp1, the chromatin association of the Psf2 subunit of the GINS complex is not affected by Cdc23 inactivation.
conclusionAn important function of Cdc23 in the elongation step of DNA replication may be to assist in the docking of DNA polymerase alpha-primase to chromatin.
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