Evidence map›Paper›PMID 15940464›Full record

ArticlePlanta2005

A methionine synthase homolog is associated with secretory vesicles in tobacco pollen tubes.

Alessandra Moscatelli, Monica Scali, Cristina Prescianotto-Baschong, Myriam Ferro, Jerome Garin, Rita Vignani, Fabrizio Ciampolini, Mauro Cresti

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 10 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 3 countries.

Alessandra MoscatelliDipartimento di Biologia, Università degli Studi di Milano, Via Celoria 26, 20133, Milano, Italy.
Monica Scali
Cristina Prescianotto-Baschong
Myriam Ferro
Jerome Garin
Rita Vignani
Fabrizio Ciampolini
Mauro Cresti
University of Siena · ITCommissariat à l'Énergie Atomique et aux Énergies Alternatives · FRLaboratoire de Chimie et Biologie des Métaux · FRUniversity of Basel · CHUniversity of Milan · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Seven isoforms of 85 kDa polypeptides (p85) were identified as methionine synthase (MetE) homologs by partial aminoacid sequencing in tobacco pollen tube extracts. Immunocytochemistry data showed a localization of the antigen on the surface of tip-focussed post-Golgi secretory vesicles (SVs), that appear to be partially associated with microtubules (Mts). The chemical dissection of pollen tube high speed supernatant (HSS) showed that two distinct pools of MetE are present in pollen tubes, one being the more acidic isoforms sedimenting at 15S and the remaining at 4S after zonal centrifugation through a sucrose density gradient. The identification of the MetE within the pollen tube and its possible participation as methyl donor in a wide range of metabolic reactions, makes it a good subject for studies on pollen tube growth regulation.

Indexed as

5-Methyltetrahydrofolate-Homocysteine S-MethyltransferaseAmino Acid SequenceIsoenzymesMolecular Sequence DataNicotianaPlant ProteinsPollenSecretory VesiclesSequence Homology, Amino Acid5-Methyltetrahydrofolate-Homocysteine S-MethyltransferaseIsoenzymesPlant Proteins

Identifiers

PMID15940464
OpenAlexW2094944764

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.