ArticleOncogene2005
Stabilization of PML nuclear localization by conjugation and oligomerization of SUMO-3.
Article in Oncogene, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 114 citations in OpenAlex.
- Patching up the nucleus: a novel role for PMLII in nuclear envelope stability.Nucleic acids research · 2026Article
- SUMO2 promotes histone pre-mRNA processing by stabilizing histone locus body interactions and facilitating U7 snRNP assembly.Genes & development · 2025Article
- UBC9-Mediated SUMO Pathway Drives Prohibitin-1 Nuclear Accumulation and PITX1 Repression in Primary Osteoarthritis.International journal of molecular sciences · 2025Article
- SUMOylation of MFF coordinates fission complexes to promote stress-induced mitochondrial fragmentation.Science advances · 2024Article
- On the Prevalence and Roles of Proteins Undergoing Liquid-Liquid Phase Separation in the Biogenesis of PML-Bodies.Biomolecules · 2023Article
- Protein-Protein Interactions Facilitate E4orf6-Dependent Regulation of E1B-55K SUMOylation in HAdV-C5 Infection.Viruses · 2022Article
- SUMOylation regulates the number and size of promyelocytic leukemia-nuclear bodies (PML-NBs) and arsenic perturbs SUMO dynamics on PML by insolubilizing PML in THP-1 cells.Archives of toxicology · 2022Article
- Effects of arsenic on the topology and solubility of promyelocytic leukemia (PML)-nuclear bodies.PloS one · 2022Article
- Chromokinesin KIF4A teams up with stathmin 1 to regulate abscission in a SUMO-dependent manner.Journal of cell science · 2020Article
- Article
- Noncovalent structure of SENP1 in complex with SUMO2.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Mad1 destabilizes p53 by preventing PML from sequestering MDM2.Nature communications · 2019Article
- PML is recruited to heterochromatin during S phase and represses DAXX-mediated histone H3.3 chromatin assembly.Journal of cell science · 2019Article
- Single-dose radiotherapy disables tumor cell homologous recombination via ischemia/reperfusion injury.The Journal of clinical investigation · 2019Article
- The Roles of SUMO in Metabolic Regulation.Advances in experimental medicine and biology · 2017Review
- SUMO5, a Novel Poly-SUMO Isoform, Regulates PML Nuclear Bodies.Scientific reports · 2016Article
- Analysis of Small Ubiquitin-Like Modifier (SUMO) Targets Reflects the Essential Nature of Protein SUMOylation and Provides Insight to Elucidate the Role of SUMO in Plant Development.Plant physiology · 2015Article
- SUMO proteomics to decipher the SUMO-modified proteome regulated by various diseases.Proteomics · 2015Review
- The function, regulation and therapeutic implications of the tumor suppressor protein, PML.Cell & bioscience · 2015Review
- Sp100 isoform-specific regulation of human adenovirus 5 gene expression.Journal of virology · 2014Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
The PML gene of acute promyelocytic leukemia (APL) encodes a cell-growth and tumor suppressor. PML localizes to discrete nuclear bodies (NBs) that are disrupted in APL cells, resulting from a reciprocal chromosome translocation t (15;17). Here we show that the nuclear localization of PML is also regulated by SUMO-3, one of the three recently identified SUMO isoforms in human cells. SUMO-3 bears similar subcellular distribution to those of SUMO-1 and -2 in the interphase nuclear body, which is colocalized with PML protein. However, both SUMO-2 and -3 are also localized to nucleoli, a region lacking SUMO-1. Immunoprecipitated PML protein bears SUMO-3 moiety in a covalently modified form, supporting the notion that PML is conjugated by SUMO-3. To determine the functional relevance of SUMO-3 conjugation on PML molecular dynamics, we suppressed SUMO-3 protein expression using a siRNA-mediated approach. Depletion of SUMO-3 markedly reduced the number of PML-containing NBa and their integrity, which is rescued by exogenous expression of SUMO-3 but not SUMO-1 or SUMO-2. The specific requirement of SUMO-3 for PML nuclear localization is validated by expression of SUMO-3 conjugation defective mutant. Moreover, we demonstrate that oligomerization of SUMO-3 is required for PML retention in the nucleus. Taken together, our studies provide first line of evidence showing that SUMO-3 is essential for PML localization and offer novel insight into the pathobiochemistry of APL.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.