ArticleProceedings of the National Academy of Sciences of the United States of America2005
Actin polymerization kinetics, cap structure, and fluctuations.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Regulating Promiscuous Catalysis via Substrate-Induced Transient Assembly.Angewandte Chemie (International ed. in English) · 2026Article
- A five-domain functional framework for integrative physiology: a coherent and operational approach to whole-body function.Frontiers in physiology · 2026Review
- ATP-dependent actin barbed-end fluctuations at steady state.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A generalized theoretical framework to investigate multicomponent actin dynamics.PLoS computational biology · 2025Article
- Hydrolysis-dependent severing tunes internal monomeric heterogeneity to shape actin length distributions.bioRxiv : the preprint server for biology · 2025Article
- Polymer dynamics of Alp7A reveals how two critical concentrations govern assembly of dynamically unstable actin-like proteins.Molecular biology of the cell · 2024Article
- Leading edge maintenance in migrating cells is an emergent property of branched actin network growth.eLife · 2022Article
- Effects of random hydrolysis on biofilament length distributions in a shared subunit pool.Biophysical journal · 2022Article
- Celebrating 20 years of live single-actin-filament studies with five golden rules.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Actin Dynamics as a Multiscale Integrator of Cellular Guidance Cues.Frontiers in cell and developmental biology · 2022Article
- Adhesion strength and contractility enable metastatic cells to become adurotactic.Cell reports · 2021Article
- Insights into Actin Polymerization and Nucleation Using a Coarse-Grained Model.Biophysical journal · 2020Article
- A stochastic model for dynamics of FtsZ filaments and the formation of Z -ring.The European physical journal. E, Soft matter · 2020Article
- Ofloxacin as a Disruptor of Actin Aggresome "Hirano Bodies": A Potential Repurposed Drug for the Treatment of Neurodegenerative Diseases.Frontiers in aging neuroscience · 2020Article
- Multiscale simulation of actin filaments and actin-associated proteins.Biophysical reviews · 2018Review
- Insights into the Cooperative Nature of ATP Hydrolysis in Actin Filaments.Biophysical journal · 2018Article
- Actin Cytoskeleton Affects Schwann Cell Migration and Peripheral Nerve Regeneration.Frontiers in physiology · 2018Article
- Steric Effects Induce Geometric Remodeling of Actin Bundles in Filopodia.Biophysical journal · 2016Article
- Actomyosin contraction, aggregation and traveling waves in a treadmilling actin array.Physica D. Nonlinear phenomena · 2016Article
- Profilin Interaction with Actin Filament Barbed End Controls Dynamic Instability, Capping, Branching, and Motility.Developmental cell · 2016Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Polymerization of actin proteins into dynamic structures is essential to eukaryotic cell life, motivating many in vitro experiments measuring polymerization kinetics of individual filaments. Here, we model these kinetics, accounting for all relevant steps revealed by experiment: polymerization, depolymerization, random ATP hydrolysis, and release of phosphate (P(i)). We relate filament growth rates to the dynamics of ATP-actin and ADP-P(i)-actin caps that develop at filament ends. At the critical concentration of the barbed end, c(crit), we find a short ATP cap and a long fluctuation-stabilized ADP-P(i) cap. We show that growth rates and the critical concentration at the barbed end are intimately related to cap structure and dynamics. Fluctuations in filament lengths are described by the length diffusion coefficient, D. Recently Fujiwara et al. [Fujiwara, I., Takahashi, S., Takaduma, H., Funatsu, T. & Ishiwata, S. (2002) Nat. Cell Biol. 4, 666-673] and Kuhn and Pollard [Kuhn, J. & Pollard, T. D. (2005) Biophys. J. 88, 1387-1402] observed large length fluctuations slightly above c(crit), provoking speculation that growth may proceed by oligomeric rather than monomeric on-off events. For the single-monomer growth process, we find that D exhibits a pronounced peak below c(crit), due to filaments alternating between capped and uncapped states, a mild version of the dynamic instability of microtubules. Fluctuations just above c(crit) are enhanced but much smaller than those reported experimentally. Future measurements of D as a function of concentration can help identify the origin of the observed fluctuations.
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