ArticleClinical and diagnostic laboratory immunology2005
Evaluation of PCR-based assay for diagnosis of spotted fever group rickettsiosis in human serum samples.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 98 citations in OpenAlex.
- Improved Detection and Sequencing of Rickettsia spp. DNA in South African Wildlife.Veterinary medicine and science · 2026Article
- Metagenomic surveillance of tick-borne pathogens and microbiomes in Huntingdon County, Pennsylvania.One health (Amsterdam, Netherlands) · 2026Article
- The mosaic of Rickettisae from the Caatinga Biome, Brazil: New records and first interactions.PLoS neglected tropical diseases · 2025Article
- Vector-borne pathogens in cats and associated fleas in southern Ethiopia.Parasites & vectors · 2025Article
- First Molecular Detection ofPathogens (Basel, Switzerland) · 2025Article
- Defining the bacterial microbiome of ticks in Chongwe and Chisamba Districts of Zambia.Infectious medicine · 2024Article
- Detection of tick-borne bacterial DNA (Rickettsia sp.) in reptile ticks Amblyomma moreliae from New South Wales, Australia.Parasitology research · 2024Article
- Molecular detection and phylogenetic characterization ofScience in One Health · 2024Article
- First Records of Possibly Human PathogenicMicroorganisms · 2023Article
- High Prevalence of Tick-Borne ZoonoticAnimals : an open access journal from MDPI · 2022Article
- Impact of Different Anthropogenic Environments on Ticks and Tick-Associated Pathogens in Alsace, a French Region Highly Endemic for Tick-Borne Diseases.Microorganisms · 2022Article
- HumanFrontiers in cellular and infection microbiology · 2022Article
- An Update on the Laboratory Diagnosis ofPathogens (Basel, Switzerland) · 2021Review
- Article
- Clinical Aspects and Detection of Emerging Rickettsial Pathogens: A "One Health" Approach Study in Serbia, 2020.Frontiers in microbiology · 2021Article
- Development of a Multiplex Loop-Mediated Isothermal Amplification (LAMP) Method for Simultaneous Detection of Spotted Fever Group Rickettsiae and Malaria Parasites by Dipstick DNA Chromatography.Diagnostics (Basel, Switzerland) · 2020Article
- New Real-Time PCRs to DifferentiateMolecules (Basel, Switzerland) · 2020Article
- Upscaling the Surveillance of Tick-borne Pathogens in the French Caribbean Islands.Pathogens (Basel, Switzerland) · 2020Article
- First records of tick-borne pathogens in populations of the taiga tick Ixodes persulcatus in Sweden.Parasites & vectors · 2019Article
- Diagnosis of spotted fever groupEpidemiology and infection · 2019Review
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nested PCR assay was developed for the detection of spotted fever group (SFG) rickettsiae in serum samples. The assay was based on specific primers derived from the rickettsial outer membrane protein B gene (rompB) of Rickettsia conorii. An SFG rickettsia-specific signal is obtained from R. akari, R. japonica, R. sibirica, and R. conorii. Other bacterial species tested did not generate any signal, attesting to the specificity of the assay. As few as seven copies of the rompB gene of R. conorii could be detected in 200 microl of serum sample. The assay was evaluated with a panel of sera obtained from patients with acute-phase febrile disease tested by immunofluorescent antibody assay (IFA). The SFG rickettsia-specific DNA fragment was detected in 71 out of 100 sera, which were proven to have immunoglobulin M antibodies against SFG rickettsial antigen by IFA. The results were further confirmed by restriction fragment length polymorphism and sequencing analysis of the DNA fragments. The results indicated that this PCR assay is suitable for the diagnosis of spotted fever group rickettsiosis in Korea.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.