ArticleNucleic acids research2005
Direct molecular haplotyping by melting curve analysis of hybridization probes: beta 2-adrenergic receptor haplotypes as an example.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
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- Highly Sensitive Detection of PIK3CA Mutations by Looping-Out Probes-Based Melting Curve Analysis.Biochemical genetics · 2024Article
- Oligonucleotide hybridization with magnetic separation assay for multiple SNP phasing.Analytica chimica acta: X · 2020Article
- Triplex real-time PCR melting curve analysis for detecting Mycobacterium tuberculosis mutations associated with resistance to second-line drugs in a single reaction.The Journal of antimicrobial chemotherapy · 2013Article
- Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.Nucleic acids research · 2013Article
- Multiplex fluorescence melting curve analysis for mutation detection with dual-labeled, self-quenched probes.PloS one · 2011Article
- A novel multiplex real-time RT-PCR assay with FRET hybridization probes for the detection and quantitation of 13 respiratory viruses.Journal of virological methods · 2010Article
- Using patient DNA to optimize therapy in heart failure patients: a move toward perfection.Heart failure reviews · 2010Article
- Search for genetic markers and functional variants involved in the development of opiate and cocaine addiction and treatment.Annals of the New York Academy of Sciences · 2010Review
- LightCycler technology in molecular diagnostics.The Journal of molecular diagnostics : JMD · 2009Article
- A comprehensive review of the genetics of juvenile idiopathic arthritis.Pediatric rheumatology online journal · 2008Article
- MDM2 gene SNP309 T/G and p53 gene SNP72 G/C do not influence diffuse large B-cell non-Hodgkin lymphoma onset or survival in central European Caucasians.BMC cancer · 2008Article
- An efficient method for multi-locus molecular haplotyping.Nucleic acids research · 2007Article
- Lack of association between beta 2-adrenergic receptor polymorphisms and juvenile idiopathic arthritis.Scandinavian journal of rheumatologyArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Direct determination of the association of multiple genetic polymorphisms, or haplotyping, in individual samples is challenging because of chromosome diploidy. Here, we describe the ability of hybridization probes, commonly used as genotyping tools, to establish single nucleotide polymorphism (SNP) haplotypes in a single step. Three haplotypes found in the beta 2-adrenergic receptor (beta2AR) gene and characterized by three different SNPs combinations are presented as examples. Each combination of SNPs has a unique stability, recorded by its melting temperature, even when intervening sequences from the template must loop out during probe hybridization. In the course of this study, two haplotypes in beta2AR not described previously were discovered. This approach provides a tool for molecular haplotyping that should prove useful in clinical molecular genetics diagnostics and pharmacogenetic research where methods for direct haplotyping are needed.
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