ArticleJournal of bacteriology2005
Analysis of Pseudomonas putida KT2440 gene expression in the maize rhizosphere: in vivo [corrected] expression technology capture and identification of root-activated promoters.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 126 citations in OpenAlex.
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- Nanoparticles: Weighing the Pros and Cons from an Eco-genotoxicological Perspective.Journal of cancer prevention · 2021Review
- Beyond the Wall: Exopolysaccharides in the Biofilm Lifestyle of Pathogenic and Beneficial Plant-AssociatedMicroorganisms · 2021Review
- Rhizosphere plant-microbe interactions under water stress.Advances in applied microbiology · 2021Article
- Root Exudates Alter the Expression of Diverse Metabolic, Transport, Regulatory, and Stress Response Genes in RhizosphereFrontiers in microbiology · 2021Article
- Construction and characterization of broad-host-range reporter plasmid suitable for on-line analysis of bacterial host responses related to recombinant protein production.Microbial cell factories · 2019Article
- Response of the Biocontrol Agent Pseudomonas pseudoalcaligenes AVO110 to Rosellinia necatrix Exudate.Applied and environmental microbiology · 2019Article
- The promoter region of lapA and its transcriptional regulation by Fis in Pseudomonas putida.PloS one · 2017Article
- Disruption of the carA gene in Pseudomonas syringae results in reduced fitness and alters motility.BMC microbiology · 2016Article
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- Restoration of a Mediterranean forest after a fire: bioremediation and rhizoremediation field-scale trial.Microbial biotechnology · 2015Article
- Polymyxin resistance of Pseudomonas aeruginosa phoQ mutants is dependent on additional two-component regulatory systems.Antimicrobial agents and chemotherapy · 2013Article
- In vivo gene expression of Pseudomonas putida KT2440 in the rhizosphere of different plants.Microbial biotechnology · 2013Article
- Comparative genomic analysis of four representative plant growth-promoting rhizobacteria in Pseudomonas.BMC genomics · 2013Article
- Gut and root microbiota commonalities.Applied and environmental microbiology · 2013Review
- Article
- Identification of traits shared by rhizosphere-competent strains of fluorescent pseudomonads.Microbial ecology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pseudomonas putida KT2440, a paradigm organism in biodegradation and a good competitive colonizer of the maize rhizosphere, was the subject of studies undertaken to establish the genetic determinants important for its rhizospheric lifestyle. By using in vivo expression technology (IVET) to positively select single cell survival, we identified 28 rap genes (root-activated promoters) preferentially expressed in the maize rhizosphere. The IVET system had two components: a mutant affected in aspartate-beta-semialdehyde dehydrogenase (asd), which was unable to survive in the rhizosphere, and plasmid pOR1, which carries a promoter-less asd gene. pOR1-borne transcriptional fusions of the rap promoters to the essential gene asd, which were integrated into the chromosome at the original position of the corresponding rap gene, were active and allowed growth of the asd strain in the rhizosphere. The fact that five of the rap genes identified in the course of this work had been formerly characterized as being related to root colonization reinforced the IVET approach. Up to nine rap genes encoded proteins either of unknown function or that had been assigned an unspecific role based on conservation of the protein family domains. Rhizosphere-induced fusions included genes with probable functions in the cell envelope, chemotaxis and motility, transport, secretion, DNA metabolism and defense mechanism, regulation, energy metabolism, stress, detoxification, and protein synthesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.