ArticleThe Journal of membrane biology1992
Effects of osmotic stress on mast cell vesicles of the beige mouse.
Article in The Journal of membrane biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Monitoring the Effect of Osmotic Stress on Secretory Vesicles and Exocytosis.Journal of visualized experiments : JoVE · 2018Article
- Mechanics of post-fusion exocytotic vesicle.Physical biology · 2017Article
- Impact of Chromogranin A deficiency on catecholamine storage, catecholamine granule morphology and chromaffin cell energy metabolism in vivo.Cell and tissue research · 2016Article
- Protein mobility within secretory granules.Biophysical journal · 2014Article
- Cholesterol accumulation increases insulin granule size and impairs membrane trafficking.Traffic (Copenhagen, Denmark) · 2012Article
- High vapor pressure perfluorocarbons cause vesicle fusion and changes in membrane packing.Biophysical journal · 2008Article
- Secretory vesicles membrane area is regulated in tandem with quantal size in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Stimulation-dependent regulation of the pH, volume and quantal size of bovine and rodent secretory vesicles.The Journal of physiology · 2002Article
- VMAT-Mediated changes in quantal size and vesicular volume.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Stimulus-secretion coupling in neurohypophysial nerve endings: a role for intravesicular sodium?Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Kinetics of release of serotonin from isolated secretory granules. II. Ion exchange determines the diffusivity of serotonin.Biophysical journal · 1997Article
- Atomic force microscopy study of the secretory granule lumen.Biophysical journal · 1996Article
- Patch clamp studies of single intact secretory granules.Biophysical journal · 1993Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The large size of the vesicles of beige mouse peritoneal mast cells (4 microns in diameter) facilitated the direct observation of the individual osmotic behavior of vesicles. The vesicle diameter increased as much as 73% when intact cells were perfused with a 10 mM pH buffer solution; the swelling of the vesicle membranes exceeded that of the insoluble vesicle gel matrix, which resulted in the formation of a clear space between the optically dense gel matrix and the vesicle membrane. Hypertonic solutions shrank intact vesicles of lysed cells in a nonideal manner, suggesting a limit to the compressibility of the gel matrix. The nonideality at high osmotic strengths can be adequately explained as the consequence of an excluded volume and/or a three-dimensional gel-matrix spring. The observed osmotic activity of the vesicles implies that the great majority of the histamine known to be present is reversibly bound to the gel matrix. This binding allows vesicles to store a large quantity of transmitter without doing osmotic work. The large size of the vesicles also facilitated the measurement of the kinetics of release as a collection of individual fusion events. Capacitance measurements in beige mast cells revealed little difference in the kinetics of release in hypotonic, isotonic, and hypertonic solutions, thus eliminating certain classes of models based on the osmotic theory of exocytosis for mast cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.