ArticleApplied and environmental microbiology2005
Microplate subtractive hybridization to enrich for bacteroidales genetic markers for fecal source identification.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- An overview of molecular markers for identification of non-human fecal pollution sources.Frontiers in microbiology · 2023Review
- Monitoring Urban Beach Quality on a Summer Day: Determination of the Origin of Fecal Indicator Bacteria and Antimicrobial Resistance at Prophète Beach, Marseille (France).Frontiers in microbiology · 2021Article
- Application of a Faecalibacterium 16S rDNA genetic marker for species identification of dog fecal waste.Environmental science and pollution research international · 2020Article
- Article
- Enterococcal Concentrations in a Coastal Ecosystem Are a Function of Fecal Source Input, Environmental Conditions, and Environmental Sources.Applied and environmental microbiology · 2018Article
- Distribution and Differential Survival of Traditional and Alternative Indicators of Fecal Pollution at Freshwater Beaches.Applied and environmental microbiology · 2017Article
- Identification of Specialists and Abundance-Occupancy Relationships among Intestinal Bacteria of Aves, Mammalia, and Actinopterygii.Applied and environmental microbiology · 2015Article
- Genetic markers for rapid PCR-based identification of gull, Canada goose, duck, and chicken fecal contamination in water.Applied and environmental microbiology · 2012Article
- Decay of bacterial pathogens, fecal indicators, and real-time quantitative PCR genetic markers in manure-amended soils.Applied and environmental microbiology · 2011Article
- Molecular identification of fecal pollution sources in water supplies by host-specific fecal DNA markers and Terminal Restriction Fragment Length Polymorphism profiles of 16S rRNA gene.Journal of microbiology (Seoul, Korea) · 2008Article
- Quantitative PCR for detection and enumeration of genetic markers of bovine fecal pollution.Applied and environmental microbiology · 2008Article
- Determining sources of fecal bacteria in waterways.Environmental monitoring and assessment · 2007Article
- High-throughput and quantitative procedure for determining sources of Escherichia coli in waterways by using host-specific DNA marker genes.Applied and environmental microbiology · 2007Article
- Basin-wide analysis of the dynamics of fecal contamination and fecal source identification in Tillamook Bay, Oregon.Applied and environmental microbiology · 2006Article
- Persistence and growth of fecal Bacteroidales assessed by bromodeoxyuridine immunocapture.Applied and environmental microbiology · 2006Article
- Development of goose- and duck-specific DNA markers to determine sources of Escherichia coli in waterways.Applied and environmental microbiology · 2006Article
- Host distributions of uncultivated fecal Bacteroidales bacteria reveal genetic markers for fecal source identification.Applied and environmental microbiology · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The ability to identify sources of fecal pollution plays a key role in the analysis of human health risk and the implementation of water resource management strategies. One approach to this problem involves the identification of bacterial lineages or gene sequences that are found exclusively in a particular host species or group. We used subtractive hybridization to enrich for target host-specific fecal Bacteroidales rRNA gene fragments that were different from those of very closely related reference (subtracter) host sources. Target host rRNA gene fragments were hybridized to subtracter rRNA gene fragments immobilized in a microplate well, and target sequences that did not hybridize were cloned and sequenced for PCR primer design. The use of microplates for DNA immobilization resulted in a one-step subtractive hybridization in which the products could be directly amplified with PCR. The new host-specific primers designed from subtracted target fragments differentiated among very closely related Bacteroidales rRNA gene sequences and distinguished between similar fecal sources, such as elk and cow or human and domestic pet (dog).
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