ArticleApplied and environmental microbiology2005
Aspergillus parasiticus cyclase catalyzes two dehydration steps in aflatoxin biosynthesis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Identification and Detection of AflatoxigenicToxins · 2026Review
- Recent advances in microbially derived chlorinated antiparasitic compounds.Molecular diversity · 2025Review
- Mycotoxin Contamination: Occurrence, Biotransformation, Pathogenic Mechanisms, and Strategies for Nutritional Intervention.Molecules (Basel, Switzerland) · 2025Review
- Current Insights in Fungal Importance-A Comprehensive Review.Microorganisms · 2023Review
- Review
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- Aflatoxin Biosynthesis, Genetic Regulation, Toxicity, and Control Strategies: A Review.Journal of fungi (Basel, Switzerland) · 2021Review
- An Unconventional Melanin Biosynthesis Pathway in Ustilago maydis.Applied and environmental microbiology · 2021Article
- Characteristics, Occurrence, Detection and Detoxification of Aflatoxins in Foods and Feeds.Foods (Basel, Switzerland) · 2020Review
- Review
- Oxidative Cyclization in Natural Product Biosynthesis.Chemical reviews · 2017Review
- Biosynthesis of oxygen and nitrogen-containing heterocycles in polyketides.Beilstein journal of organic chemistry · 2016Review
- Review
- Biosynthesis of dothistromin.Mycopathologia · 2006Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the aflatoxin biosynthetic pathway, 5'-oxoaverantin (OAVN) cyclase, the cytosolic enzyme, catalyzes the reaction from OAVN to (2'S,5'S)-averufin (AVR) (E. Sakuno, K. Yabe, and H. Nakajima, Appl. Environ. Microbiol. 69:6418-6426, 2003). Interestingly, the N-terminal 25-amino-acid sequence of OAVN cyclase completely matched an internal sequence of the versiconal (VHOH) cyclase that was deduced from its gene (vbs). The purified OAVN cyclase also catalyzed the reaction from VHOH to versicolorin B (VB). In a competition experiment using the cytosol fraction of Aspergillus parasiticus, a high concentration of VHOH inhibited the enzyme reaction from OAVN to AVR, and instead VB was newly formed. The recombinant Vbs protein, which was expressed in Pichia pastoris, showed OAVN cyclase activity, as well as VHOH cyclase activity. A mutant of A. parasiticus SYS-4 (= NRRL 2999) with vbs deleted accumulated large amounts of OAVN, 5'-hydroxyaverantin, averantin, AVR, and averufanin in the mycelium. These results indicated that the cyclase encoded by the vbs gene is also involved in the reaction from OAVN to AVR in aflatoxin biosynthesis. Small amounts of VHOH, VB, and aflatoxins also accumulated in the same mutant, and this accumulation may have been due to an unknown enzyme(s) not involved in aflatoxin biosynthesis. This is the first report of one enzyme catalyzing two different reactions in a pathway of secondary metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.