ArticleApplied and environmental microbiology2005
Real-time quantitative reverse transcription-PCR analysis of expression stability of Actinobacillus pleuropneumoniae housekeeping genes during in vitro growth under iron-depleted conditions.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 54 citations in OpenAlex.
- Identification of stable reference genes in Edwardsiella ictaluri for accurate gene expression analysis.PloS one · 2025Article
- Transcriptomic and proteomic profiling ofFrontiers in cellular and infection microbiology · 2025Article
- Tools to study microbial iron homeostasis and oxidative stress: current techniques and methodological gaps.Frontiers in molecular biosciences · 2025Review
- Abundant and persistent sulfur-oxidizing microbial populations are responsive to hypoxia in the Chesapeake Bay.Environmental microbiology · 2022Article
- Molecular characterization of stress tolerance genes associated with D. indicus strain under extreme environment conditions.Environmental geochemistry and health · 2021Article
- Selection and validation of reference genes for gene expression studies in Klebsiella pneumoniae using Reverse Transcription Quantitative real-time PCR.Scientific reports · 2018Article
- The N-linking glycosylation system from Actinobacillus pleuropneumoniae is required for adhesion and has potential use in glycoengineering.Open biology · 2017Article
- The SapA Protein Is Involved in Resistance to Antimicrobial Peptide PR-39 and Virulence ofFrontiers in microbiology · 2017Article
- Pyridoxal phosphate synthases PdxS/PdxT are required for Actinobacillus pleuropneumoniae viability, stress tolerance and virulence.PloS one · 2017Article
- Quantification of the Genetic Expression of bgl-A, bgl, and CspA and Enzymatic Characterization of β-Glucosidases from Shewanella sp. G5.Marine biotechnology (New York, N.Y.) · 2016Article
- Comparative proteomics: assessment of biological variability and dataset comparability.BMC bioinformatics · 2015Article
- Validation of reference genes for quantitative real-time PCR (qPCR) analysis of Actinobacillus suis.BMC research notes · 2015Article
- Role of (p)ppGpp in Viability and Biofilm Formation of Actinobacillus pleuropneumoniae S8.PloS one · 2015Article
- Article
- Evaluation of reference genes for gene expression analysis using quantitative RT-PCR in Azospirillum brasilense.PloS one · 2014Article
- Evaluation of reference genes for reverse transcription quantitative PCR analyses of fish-pathogenic Francisella strains exposed to different growth conditions.BMC research notes · 2013Article
- Evaluation of suitable reference genes for gene expression studies in porcine PBMCs in response to LPS and LTA.BMC research notes · 2013Article
- Article
- Comparative profiling of the transcriptional response to iron restriction in six serotypes of Actinobacillus pleuropneumoniae with different virulence potential.BMC genomics · 2010Article
- Screen for leukotoxin mutants in Aggregatibacter actinomycetemcomitans: genes of the phosphotransferase system are required for leukotoxin biosynthesis.Infection and immunity · 2008Article
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2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The aims of the present investigation were to develop and test a sensitive and reproducible method for the study of gene expression in the porcine lung pathogen Actinobacillus pleuropneumoniae by real-time quantitative reverse transcription (RT)-PCR and to evaluate a number of suitable internal controls, as such controls have not been defined yet for this bacterium. Bacterial gene expression was studied during in vitro exponential and early stationary growth in medium with and without sufficient iron, respectively. First, the stability of expression of five genes, the glyA, tpiA, pykA, recF, and rhoAP genes involved in basic housekeeping, was evaluated on the basis of the mean pairwise variation. All the housekeeping genes included were stably expressed under the conditions investigated and consequently were included in the normalization procedure. Next, the geometric mean of the internal control genes was used to correct five genes of interest. These genes were three genes involved in iron acquisition (tbpA, exbB, and fhuD), the heat shock protein gene groEL, and a putative quorum-sensing gene (luxS). The level of tbpA, exbB, and fhuD expression in A. pleuropneumoniae showed significant up-regulation under iron-restricted conditions compared to bacteria grown in medium with sufficient iron. The observed expression patterns of the genes of interest were consistent with previous observations. This study therefore lends further support to the use of real-time quantitative RT-PCR, with the glyA, tpiA, pykA, recF, and rhoAP genes as internal controls, for future similar gene expression studies in A. pleuropneumoniae.
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