ArticleApplied and environmental microbiology2005
Cloning and characterization of the glucosidase II alpha subunit gene of Trichoderma reesei: a frameshift mutation results in the aberrant glycosylation profile of the hypercellulolytic strain Rut-C30.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Transgressive phenotypes from outbreeding between theMicrobiology spectrum · 2024Article
- The protein methyltransferase TrSAM inhibits cellulase gene expression by interacting with the negative regulator ACE1 in Trichoderma reesei.Communications biology · 2024Article
- The Mechanism of Transcription Factor Swi6 in Regulating Growth and Pathogenicity ofMicroorganisms · 2023Article
- Simultaneous manipulation of multiple genes within a same regulatory stage for iterative evolution of Trichoderma reesei.Biotechnology for biofuels and bioproducts · 2022Article
- Glucose-lactose mixture feeds in industry-like conditions: a gene regulatory network analysis on the hyperproducing Trichoderma reesei strain Rut-C30.BMC genomics · 2020Article
- A Nuclear Magnetic Resonance (NMR) Platform for Real-Time Metabolic Monitoring of Bioprocesses.Molecules (Basel, Switzerland) · 2020Article
- Engineering ofBiotechnology for biofuels · 2020Article
- Enhancement of cellulase production in Trichoderma reesei RUT-C30 by comparative genomic screening.Microbial cell factories · 2019Article
- Harnessing the knowledge of protein secretion for enhanced protein production in filamentous fungi.World journal of microbiology & biotechnology · 2019Review
- Inducible promoters and functional genomic approaches for the genetic engineering of filamentous fungi.Applied microbiology and biotechnology · 2018Review
- Glycoside Hydrolase MoGls2 Controls Asexual/Sexual Development, Cell Wall Integrity and Infectious Growth in the Rice Blast Fungus.PloS one · 2016Article
- Involvement of the adaptor protein 3 complex in lignocellulase secretion in Neurospora crassa revealed by comparative genomic screening.Biotechnology for biofuels · 2015Article
- Effect of earlier unfolded protein response and efficient protein disposal system on cellulase production in Rut C30.World journal of microbiology & biotechnology · 2014Article
- N-glycomic profiling of a glucosidase II mutant of Dictyostelium discoideum by ''off-line'' liquid chromatography and mass spectrometry.Electrophoresis · 2014Article
- Systems biological approaches towards understanding cellulase production by Trichoderma reesei.Journal of biotechnology · 2013Review
- Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes.Scientific reports · 2013Article
- Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates.Microbial cell factories · 2012Article
- Quantitative secretomic analysis of Trichoderma reesei strains reveals enzymatic composition for lignocellulosic biomass degradation.Molecular & cellular proteomics : MCP · 2012Article
- Correlation of gene expression and protein production rate - a system wide study.BMC genomics · 2011Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
We describe isolation and characterization of the gene encoding the glucosidase II alpha subunit (GIIalpha) of the industrially important fungus Trichoderma reesei. This subunit is the catalytic part of the glucosidase II heterodimeric enzyme involved in the structural modification within the endoplasmic reticulum (ER) of N-linked oligosaccharides present on glycoproteins. The gene encoding GIIalpha (gls2alpha) in the hypercellulolytic strain Rut-C30 contains a frameshift mutation resulting in a truncated gene product. Based on the peculiar monoglucosylated N-glycan pattern on proteins produced by the strain, we concluded that the truncated protein can still hydrolyze the first alpha-1,3-linked glucose residue but not the innermost alpha-1,3-linked glucose residue from the Glc2Man9GlcNAc2 N-glycan ER structure. Transformation of the Rut-C30 strain with a repaired T. reesei gls2alpha gene changed the glycosylation profile significantly, decreasing the amount of monoglucosylated structures and increasing the amount of high-mannose N-glycans. Full conversion to high-mannose carbohydrates was not obtained, and this was probably due to competition between the endogenous mutant subunit and the introduced wild-type GIIalpha protein. Since glucosidase II is also involved in the ER quality control of nascent polypeptide chains, its transcriptional regulation was studied in a strain producing recombinant tissue plasminogen activator (tPA) and in cultures treated with the stress agents dithiothreitol (DTT) and brefeldin A (BFA), which are known to block protein transport and to induce the unfolded protein response. While the mRNA levels were clearly upregulated upon tPA production or BFA treatment, no such enhancement was observed after DTT addition.
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