ArticleApplied and environmental microbiology2005
Lactobacillus casei DN-114 001 inhibits the ability of adherent-invasive Escherichia coli isolated from Crohn's disease patients to adhere to and to invade intestinal epithelial cells.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of a Fermented Dairy Drink ContainingNutrients · 2020Pooled it
- Efficacy of Lactobacillus casei treatment on small bowel injury in chronic low-dose aspirin users: a pilot randomized controlled study.Journal of gastroenterology · 2011Trial
- Use of a fermented dairy probiotic drink containing Lactobacillus casei (DN-114 001) to decrease the rate of illness in kids: the DRINK study. A patient-oriented, double-blind, cluster-randomized, placebo-controlled, clinical trial.European journal of clinical nutrition · 2010Trial
- High thrombin activity associated with crohn's disease induces microbiota pathogenicity contributing to mucosal inflammation.Gut microbes · 2026Article
- Infant-derivedFrontiers in nutrition · 2026Article
- Ion transport and epithelial barrier dysfunction in experimental models of ulcerative colitis.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- The Efficacy of Selected Probiotic Strains and Their Combination to Inhibit the Interaction of Adherent-InvasiveMicroorganisms · 2024Article
- Association between gut microbiota and pan-dermatological diseases: a bidirectional Mendelian randomization research.Frontiers in cellular and infection microbiology · 2024Article
- The Sdp-SH3b2 domain contained inFrontiers in immunology · 2024Article
- Evaluation of the Efficacy of Xyloglucan, Pea Protein andInternational journal of molecular sciences · 2023Article
- Combined Therapy of Probiotic Microcapsules and Bomidin inLife (Basel, Switzerland) · 2022Article
- Efficacy of Selected Live Biotherapeutic Candidates to Inhibit the Interaction of an Adhesive-InvasiveBiomedicines · 2022Article
- Comparative analysis of mRNA transcripts of HT-29 cell line expressed in identical quantities for pathogenicJournal of genomics · 2022Article
- Impact of Food-Derived Bioactive Compounds on Intestinal Immunity.Biomolecules · 2021Review
- Effects of Antibiotics upon the Gut Microbiome: A Review of the Literature.Biomedicines · 2020Review
- Article
- Establishment and Application of Peristaltic Human Gut-Vessel Microsystem for Studying Host-Microbial Interaction.Frontiers in bioengineering and biotechnology · 2020Article
- Evaluation of Lactic Acid Bacteria Isolated from Fermented Plant Products for Antagonistic Activity Against Urinary Tract Pathogen Staphylococcus saprophyticus.Probiotics and antimicrobial proteins · 2018Article
- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis inFrontiers in microbiology · 2018Article
- Immunomodulation of Lactobacillus rhamnosus GG (LGG)-derived soluble factors on antigen-presenting cells of healthy blood donors.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ileal lesions in 36.4% of patients with Crohn's disease are colonized by pathogenic adherent-invasive Escherichia coli. The aim of this study was to determine the in vitro inhibitory effects of the probiotic strain, Lactobacillus casei DN-114 001, on adhesion to and invasion of human intestinal epithelial cells by adherent-invasive E. coli isolated from Crohn's disease patients. The experiments were performed with undifferentiated Intestine-407 cells and with undifferentiated or differentiated Caco-2 intestinal epithelial cells. Bacterial adhesion to and invasion of intestinal epithelial cells were assessed by counting CFU. The inhibitory effects of L. casei were determined after coincubation with adherent-invasive E. coli or after preincubation of intestinal cells with L. casei prior to infection with adherent-invasive E. coli. Inhibitory effects of L. casei on adherent-invasive E. coli adhesion to differentiated and undifferentiated intestinal epithelial cells reached 75% to 84% in coincubation and 43% to 62% in preincubation experiments, according to the cell lines used. Addition of L. casei culture supernatant to the incubation medium increased L. casei adhesion to intestinal epithelial cells and enhanced the inhibitory effects of L. casei. The inhibitory effects on E. coli invasion paralleled those on adhesion. This effect was not due to a bactericidal effect on adherent-invasive E. coli or to a cytotoxic effect on epithelial intestinal cells. As Lactobacillus casei DN-114 001 strongly inhibits interaction of adherent-invasive E. coli with intestinal epithelial cells, this finding suggests that the probiotic strain could be of therapeutic value in Crohn's disease.
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