ArticleProceedings of the National Academy of Sciences of the United States of America2005
Experimental tests of a homology model for OxlT, the oxalate transporter of Oxalobacter formigenes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 25 citations in OpenAlex.
- Structure and mechanism of oxalate transporter OxlT in an oxalate-degrading bacterium in the gut microbiota.Nature communications · 2023Article
- An optogenetic assay method for electrogenic transporters using Escherichia coli co-expressing light-driven proton pump.Protein science : a publication of the Protein Society · 2021Article
- Inhibition of the Vesicular Glutamate Transporter (VGLUT) with Congo Red Analogs: New Binding Insights.Neurochemical research · 2021Article
- Relocation of active site carboxylates in major facilitator superfamily multidrug transporter LmrP reveals plasticity in proton interactions.Scientific reports · 2016Article
- The Structure and Function of OxlT, the Oxalate Transporter of Oxalobacter formigenes.The Journal of membrane biology · 2015Review
- Closure of the cytoplasmic gate formed by TM5 and TM11 during transport in the oxalate/formate exchanger from Oxalobacter formigenes.Biochemistry · 2014Article
- Oligomeric state of the oxalate transporter, OxlT.Biochemistry · 2011Article
- Death of the TonB Shuttle Hypothesis.Frontiers in microbiology · 2011Article
- Search for the acetylcholine and vesamicol binding sites in vesicular acetylcholine transporter: the region around the lumenal end of the transport channel.Journal of neurochemistry · 2010Article
- Rational mutational analysis of a multidrug MFS transporter CaMdr1p of Candida albicans by employing a membrane environment based computational approach.PLoS computational biology · 2009Article
- Cysteine scanning mutagenesis of TM5 reveals conformational changes in OxlT, the oxalate transporter of Oxalobacter formigenes.Biochemistry · 2008Article
- Ins and outs of major facilitator superfamily antiporters.Annual review of microbiology · 2008Review
- Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins.Nature protocols · 2007Article
- Analysis of substrate-binding elements in OxlT, the oxalate:formate antiporter of Oxalobacter formigenes.Biochemistry · 2006Article
- On the accuracy of homology modeling and sequence alignment methods applied to membrane proteins.Biophysical journal · 2006Article
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7 authors at 1 institution in 1 country.
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Abstract
Using the x-ray structure of the glycerol 3-phosphate transporter (GlpT), we devised a model for the distantly related oxalate transporter, OxlT. The model accommodates all earlier biochemical information on OxlT, including the idea that Lys-355 lies on the permeation pathway, and predicts that Lys-355 and a second positive center, Arg-272, comprise the binding site for divalent oxalate. Study of R272K, R272A, and R272Q derivatives verifies that Arg-272 is essential, and comparisons with GlpT show that both anion transporters bind substrates within equivalent domains. In 22 single-cysteine variants in TM7 and TM8, topology as marked by accessibility to Oregon green maleimide is predicted by the model, with similar concordance for 52 positions probed earlier. The model also reconciles cross-linking of a cysteine pair placed near the periplasmic ends of TM2 and TM7, and retrospective study of TM2 and TM11 confirms that positions supporting disulfide trapping lie at a helical interface. Our work describes a pathway to the modeling of OxlT and other transporters in the major facilitator superfamily and outlines simple experimental tests to evaluate such proposals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.