ArticleThe Journal of physiology2005
Low carbon dioxide permeability of the apical epithelial membrane of guinea-pig colon.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 59 citations in OpenAlex.
- Article
- OCellular and molecular life sciences : CMLS · 2021Article
- COJournal of enzyme inhibition and medicinal chemistry · 2021Article
- Carbon dioxide transport across membranes.Interface focus · 2021Review
- Single-cell OProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Role of Carbonic Anhydrases and Inhibitors in Acid-Base Physiology: Insights from Mathematical Modeling.International journal of molecular sciences · 2019Review
- KThe Journal of physiology · 2018Article
- Review
- Measuring CO2 and HCO3- permeabilities of isolated chloroplasts using a MIMS-18O approach.Journal of experimental botany · 2017Article
- Rectification of oxygen transfer through the rat colonic epithelium.World journal of gastrointestinal pathophysiology · 2017Article
- Bicarbonate-rich fluid secretion predicted by a computational model of guinea-pig pancreatic duct epithelium.The Journal of physiology · 2017Article
- Colon Necrosis Due to Sodium Polystyrene Sulfonate with and without Sorbitol: An Experimental Study in Rats.PloS one · 2015Article
- Mathematical modeling of acid-base physiology.Progress in biophysics and molecular biology · 2015Review
- How does carbon dioxide permeate cell membranes? A discussion of concepts, results and methods.Frontiers in physiology · 2014Review
- Activity and distribution of intracellular carbonic anhydrase II and their effects on the transport activity of anion exchanger AE1/SLC4A1.The Journal of physiology · 2013Article
- A reaction-diffusion model of CO2 influx into an oocyte.Journal of theoretical biology · 2012Article
- Gas-tight triblock-copolymer membranes are converted to CO₂ permeable by insertion of plant aquaporins.Scientific reports · 2012Article
- The mechanistic basis of internal conductance: a theoretical analysis of mesophyll cell photosynthesis and CO2 diffusion.Plant physiology · 2011Article
- A mathematical model of tumour and blood pHe regulation: The HCO3-/CO2 buffering system.Mathematical biosciences · 2011Article
- Cloning and characterization of a zebrafish homologue of human AQP1: a bifunctional water and gas channel.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have investigated the apical membrane permeability for CO2 of intact epithelia of proximal and distal colon of the guinea pig. The method used was the mass spectrometric 18O-exchange technique previously described. In a first step, we determined the intraepithelial carbonic anhydrase (CA) activity by studying vital isolated colonocytes before and after lysis with Triton X-100. Intraepithelial CA activity was found to be 41,000 and 900 for proximal and distal colon, respectively. Then 18O-exchange measurements were done with stripped intact epithelial layers, which on their apical side were exposed to the reaction solution containing 18O-labelled CO2 and HCO3-. The mass spectrometric signals in these measurements are determined by the intracellular epithelial CA activity, and by the apical membrane permeabilities for CO2 and HCO3-, P(CO2) and P(HCO3). From the signals, we calculated the two permeabilities while inserting the CA activities obtained from isolated colonocytes. From layers of intact colon epithelium, the apical P(CO2) was determined to be 1.5 x 10(-3) cm s(-1) for proximal and 0.77 x 10(-3) cm s(-1) for distal colon. These values are > or =200 times lower than the P(CO2) of the human red cell membrane as studied with the same technique (0.3 cm s(-1)). We conclude that the apical membrane offers a significant resistance towards CO2 diffusion, which implies that a major drop in CO2 partial pressure (pCO2) will occur across the apical membrane when luminal pCO2 is higher than basolateral or capillary pCO2. In view of the very high pCO2 that can occur in the colonic lumen, this property of the apical membrane constitutes a significant protection of the cell against the high acid load associated with high pCO2.
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