ArticleClinical and experimental immunology2005
Epitope analysis of insulin autoantibodies using recombinant Fab.
Article in Clinical and experimental immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- GAD autoantibody epitope pattern after GAD-alum treatment in children and adolescents with type 1 diabetes.Pediatric diabetes · 2012Trial
- Molecular Portrait of Autoantigens in Type 1 Diabetes.Biomolecules · 2025Review
- Anti-Islet Autoantibodies in Type 1 Diabetes.International journal of molecular sciences · 2023Review
- Memory IgM protects endogenous insulin from autoimmune destruction.The EMBO journal · 2021Article
- Primary Immune Responses and Affinity Maturation Are Controlled by IgD.Frontiers in immunology · 2021Article
- Etiopathogenesis of insulin autoimmunity.Anatomy research international · 2012Article
- Latent autoimmune diabetes in adults (LADA) is dead: long live autoimmune diabetes!Diabetologia · 2010Article
- Autoantibodies against appetite-regulating peptide hormones and neuropeptides: putative modulation by gut microflora.Nutrition (Burbank, Los Angeles County, Calif.) · 2008Article
- Longitudinal changes in epitope recognition of autoantibodies against glutamate decarboxylase 65 (GAD65Ab) in prediabetic adults developing diabetes.Clinical and experimental immunology · 2007Article
- Longitudinal epitope analysis of insulin-binding antibodies in type 1 diabetes.Clinical and experimental immunology · 2006Article
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Authors and funding
11 authors at 6 institutions in 3 countries.
Funding
Abstract
Autoantibodies to insulin are often the first autoantibodies detected in young children with type 1 diabetes and can be present before the onset of clinical diabetes. These autoantibodies and their epitopes are, however, not well characterized. We explored the use of monoclonal antibodies and their recombinant Fab as reagents for epitope analysis. In this study we cloned and characterized the recombinant Fab of the insulin-specific monoclonal antibody CG7C7. We found the epitope of this antibody to be located predominantly at the A-chain loop of the insulin molecule. The recombinant Fab was then used to compete for insulin binding against insulin autoantibodies present in sera from patients with type 1 or type 1.5 diabetes. In competition experiments with sera positive for autoantibodies to insulin the recombinant Fab significantly reduced the binding to [125I]-insulin by sera of type 1 (n = 35) and type 1.5 diabetes [latent autoimmune diabetes in adults (LADA)] (n = 14) patients (P < 0.0001). We conclude that competition between insulin-specific monoclonal antibodies or their recombinant Fab and insulin autoantibodies should prove useful in the epitope analysis of autoantibodies to insulin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.