ArticleGenome research2005
A BAC-based physical map of the Drosophila buzzatii genome.
Article in Genome research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed, 2 citations in OpenAlex.
- Concurrent Duplication of Drosophila Cid and Cenp-C Genes Resulted in Accelerated Evolution and Male Germline-Biased Expression of the New Copies.Journal of molecular evolution · 2018Article
- Dense gene physical maps of the non-model species Drosophila subobscura.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Article
- Construction of cytogenetic map of Gossypium herbaceum chromosome 1 and its integration with genetic maps.Molecular cytogenetics · 2015Article
- Genomics of ecological adaptation in cactophilic Drosophila.Genome biology and evolution · 2014Article
- Segmental duplication, microinversion, and gene loss associated with a complex inversion breakpoint region in Drosophila.Molecular biology and evolution · 2012Article
- Gene alterations at Drosophila inversion breakpoints provide prima facie evidence for natural selection as an explanation for rapid chromosomal evolution.BMC genomics · 2012Article
- The 19 genomes of Drosophila: a BAC library resource for genus-wide and genome-scale comparative evolutionary research.Genetics · 2011Article
- Testing chromosomal phylogenies and inversion breakpoint reuse in Drosophila. The martensis cluster revisited.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Article
- The transposon Galileo generates natural chromosomal inversions in Drosophila by ectopic recombination.PloS one · 2009Article
- A BAC-based physical map of the Hessian fly genome anchored to polytene chromosomes.BMC genomics · 2009Article
- Cloning and sequencing of the breakpoint regions of inversion 5g fixed in Drosophila buzzatii.Chromosoma · 2009Article
- Automated FingerPrint Background removal: FPB.BMC bioinformatics · 2009Article
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- Cotton genome mapping with new microsatellites from Acala 'Maxxa' BAC-ends.Molecular genetics and genomics : MGG · 2006Article
- Conservation of regulatory sequences and gene expression patterns in the disintegrating Drosophila Hox gene complex.Genome research · 2005Article
Corrections and comments
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Authors and funding
14 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-insert genomic libraries facilitate cloning of large genomic regions, allow the construction of clone-based physical maps, and provide useful resources for sequencing entire genomes. Drosophila buzzatii is a representative species of the repleta group in the Drosophila subgenus, which is being widely used as a model in studies of genome evolution, ecological adaptation, and speciation. We constructed a Bacterial Artificial Chromosome (BAC) genomic library of D. buzzatii using the shuttle vector pTARBAC2.1. The library comprises 18,353 clones with an average insert size of 152 kb and an approximately 18x expected representation of the D. buzzatii euchromatic genome. We screened the entire library with six euchromatic gene probes and estimated the actual genome representation to be approximately 23x. In addition, we fingerprinted by restriction digestion and agarose gel electrophoresis a sample of 9555 clones, and assembled them using FingerPrint Contigs (FPC) software and manual editing into 345 contigs (mean of 26 clones per contig) and 670 singletons. Finally, we anchored 181 large contigs (containing 7788 clones) to the D. buzzatii salivary gland polytene chromosomes by in situ hybridization of 427 representative clones. The BAC library and a database with all the information regarding the high coverage BAC-based physical map described in this paper are available to the research community.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.