ArticleMolecular biology of the cell2005
Spindle checkpoint signaling requires the mis6 kinetochore subcomplex, which interacts with mad2 and mitotic spindles.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Inositol Pyrophosphate Kinase Asp1 Modulates Chromosome Segregation Fidelity and Spindle Function in Schizosaccharomyces pombe.Molecular and cellular biology · 2016Article
- Article
- Condensin HEAT subunits required for DNA repair, kinetochore/centromere function and ploidy maintenance in fission yeast.PloS one · 2015Article
- Article
- Overexpression of p42.3 promotes cell growth and tumorigenicity in hepatocellular carcinoma.World journal of gastroenterology · 2013Article
- Sos7, an essential component of the conserved Schizosaccharomyces pombe Ndc80-MIND-Spc7 complex, identifies a new family of fungal kinetochore proteins.Molecular and cellular biology · 2012Article
- The ABCs of CENPs.Chromosoma · 2011Review
- CENP-A reduction induces a p53-dependent cellular senescence response to protect cells from executing defective mitoses.Molecular and cellular biology · 2010Article
- Dissection of CENP-C-directed centromere and kinetochore assembly.Molecular biology of the cell · 2009Article
- The life and miracles of kinetochores.The EMBO journal · 2009Review
- Fission yeast Ccq1 is telomerase recruiter and local checkpoint controller.Genes & development · 2008Article
- Dual regulation of Mad2 localization on kinetochores by Bub1 and Dam1/DASH that ensure proper spindle interaction.Molecular biology of the cell · 2008Article
- Schizosaccharomyces pombe Bub3 is dispensable for mitotic arrest following perturbed spindle formation.Genetics · 2008Article
- Biphasic incorporation of centromeric histone CENP-A in fission yeast.Molecular biology of the cell · 2008Article
- The CENP-A NAC/CAD kinetochore complex controls chromosome congression and spindle bipolarity.The EMBO journal · 2007Article
- Msc1 acts through histone H2A.Z to promote chromosome stability in Schizosaccharomyces pombe.Genetics · 2007Article
- The conserved Spc7 protein is required for spindle integrity and links kinetochore complexes in fission yeast.Molecular biology of the cell · 2007Article
- Mitotic chromosome biorientation in fission yeast is enhanced by dynein and a minus-end-directed, kinesin-like protein.Molecular biology of the cell · 2007Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The spindle checkpoint coordinates cell cycle progression and chromosome segregation by inhibiting anaphase promoting complex/cyclosome until all kinetochores interact with the spindle properly. During early mitosis, the spindle checkpoint proteins, such as Mad2 and Bub1, accumulate at kinetochores that do not associate with the spindle. Here, we assess the requirement of various kinetochore components for the accumulation of Mad2 and Bub1 on the kinetochore in fission yeast and show that the necessity of the Mis6-complex and the Nuf2-complex is an evolutionarily conserved feature in the loading of Mad2 onto the kinetochore. Furthermore, we demonstrated that Nuf2 is required for maintaining the Mis6-complex on the kinetochore during mitosis. The Mis6-complex physically interacts with Mad2 under the condition that the Mad2-dependent checkpoint is activated. Ectopically expressed N-terminal fragments of Mis6 localize along the mitotic spindle, highlighting the potential binding ability of Mis6 not only to the centromeric chromatin but also to the spindle microtubules. We propose that the Mis6-complex, in collaboration with the Nuf2-complex, monitors the spindle-kinetochore attachment state and acts as a platform for Mad2 to accumulate at unattached kinetochores.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.