ArticleMolecular biology of the cell2005
Inhibition of translation initiation by volatile anesthetics involves nutrient-sensitive GCN-independent and -dependent processes in yeast.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 31 citations in OpenAlex.
- Translation elongation rate varies among organs and decreases with age.Nucleic acids research · 2021Article
- Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.Biochemical and biophysical research communications · 2020Article
- Effects of the presence and absence of amino acids on translation, signaling, and long-term depression in hippocampal slices from Fmr1 knockout mice.Journal of neurochemistry · 2019Article
- Farnesol inhibits translation to limit growth and filamentation inMicrobial cell (Graz, Austria) · 2017Article
- Article
- Local Anesthetics and Antipsychotic Phenothiazines Interact Nonspecifically with Membranes and Inhibit Hexose Transporters in Yeast.Genetics · 2016Article
- Alcohols inhibit translation to regulate morphogenesis in C. albicans.Fungal genetics and biology : FG & B · 2015Article
- Tetracaine, a local anesthetic, preferentially induces translational inhibition with processing body formation rather than phosphorylation of eIF2α in yeast.Current genetics · 2015Article
- eIF2 kinases mediate β-lapachone toxicity in yeast and human cancer cells.Cell cycle (Georgetown, Tex.) · 2015Article
- Review
- Glucose depletion inhibits translation initiation via eIF4A loss and subsequent 48S preinitiation complex accumulation, while the pentose phosphate pathway is coordinately up-regulated.Molecular biology of the cell · 2011Article
- Power of yeast for analysis of eukaryotic translation initiation.The Journal of biological chemistry · 2010Review
- Fusel alcohols regulate translation initiation by inhibiting eIF2B to reduce ternary complex in a mechanism that may involve altering the integrity and dynamics of the eIF2B body.Molecular biology of the cell · 2010Article
- Discovery of chemical modulators of a conserved translational control pathway by parallel screening in yeast.Assay and drug development technologies · 2009Article
- Gcn4 is required for the response to peroxide stress in the yeast Saccharomyces cerevisiae.Molecular biology of the cell · 2008Article
- Antimicrobial effects of liquid anesthetic isoflurane on Candida albicans.Journal of translational medicine · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Volatile anesthetics including isoflurane affect all cells examined, but their mechanisms of action remain unknown. To investigate the cellular basis of anesthetic action, we are studying Saccharomyces cerevisiae mutants altered in their response to anesthetics. The zzz3-1 mutation renders yeast isoflurane resistant and is an allele of GCN3. Gcn3p functions in the evolutionarily conserved general amino acid control (GCN) pathway that regulates protein synthesis and gene expression in response to nutrient availability through phosphorylation of the alpha subunit of eukaryotic initiation factor 2 (eIF2alpha). Hyperphosphorylation of eIF2alpha inhibits translation initiation during amino acid starvation. Isoflurane rapidly (in <15 min) inhibits yeast cell division and amino acid uptake. Unexpectedly, phosphorylation of eIF2alpha decreased dramatically upon initial exposure although hyperphosphorylation occurred later. Translation initiation was inhibited by isoflurane even when eIF2alpha phosphorylation decreased and this inhibition was GCN-independent. Maintenance of inhibition required GCN-dependent hyperphosphorylation of eIF2alpha. Thus, two nutrient-sensitive stages displaying unique features promote isoflurane-induced inhibition of translation initiation. The rapid phase is GCN-independent and apparently has not been recognized previously. The maintenance phase is GCN-dependent and requires inhibition of general translation imparted by enhanced eIF2alpha phosphorylation. Surprisingly, as shown here, the transcription activator Gcn4p does not affect anesthetic response.
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