ArticleThe Biochemical journal2005
Human Rhesus B and Rhesus C glycoproteins: properties of facilitated ammonium transport in recombinant kidney cells.
Article in The Biochemical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 87 citations in OpenAlex.
- Mutations in ammonia transporter RhBG that impair NHThe Journal of physiology · 2026Article
- Rhbg interaction with CA-IV and its effects on NHAmerican journal of physiology. Cell physiology · 2025Article
- Ammonia transporter RhBG initiates downstream signaling and functional responses by activating NFκB.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Biological ammonium transporters from the Amt/Mep/Rh superfamily: mechanism, energetics, and technical limitations.Bioscience reports · 2024Review
- Effects of chronic hypercapnia on ammonium transport in the mouse kidney.Physiological reports · 2019Article
- Ammonia Transporters and Their Role in Acid-Base Balance.Physiological reviews · 2017Review
- Structural determinants of NH3 and NH4+ transport by mouse Rhbg, a renal Rh glycoprotein.American journal of physiology. Renal physiology · 2016Article
- Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins.American journal of physiology. Cell physiology · 2015Article
- Renal acid-base regulation: new insights from animal models.Pflugers Archiv : European journal of physiology · 2015Review
- Evidence of a structural and functional ammonium transporter RhBG·anion exchanger 1·ankyrin-G complex in kidney epithelial cells.The Journal of biological chemistry · 2015Article
- Ammonia excretion in Caenorhabditis elegans: mechanism and evidence of ammonia transport of the Rhesus protein CeRhr-1.The Journal of experimental biology · 2015Article
- Wnt/β-Catenin Signaling Regulates the Expression of the Ammonium Permease Gene RHBG in Human Cancer Cells.PloS one · 2015Article
- Ammonia transport in the kidney by Rhesus glycoproteins.American journal of physiology. Renal physiology · 2014Review
- Effect of collecting duct-specific deletion of both Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) on renal response to metabolic acidosis.American journal of physiology. Renal physiology · 2014Article
- Relative CO₂/NH₃ permeabilities of human RhAG, RhBG and RhCG.The Journal of membrane biology · 2013Article
- Renal ammonia metabolism and transport.Comprehensive Physiology · 2013Review
- Cystic fibrosis transmembrane conductance regulator in the gills of the climbing perch, Anabas testudineus, is involved in both hypoosmotic regulation during seawater acclimation and active ammonia excretion during ammonia exposure.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2012Article
- Expression of blood group genes by mesenchymal stem cells.British journal of haematology · 2011Article
- Role of NH3 and NH4+ transporters in renal acid-base transport.American journal of physiology. Renal physiology · 2011Review
- pH sensitivity of ammonium transport by Rhbg.American journal of physiology. Cell physiology · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mammalian Rh (Rhesus) protein family belongs to the Amt/Mep (ammonia transporter/methylammonium permease)/Rh superfamily of ammonium transporters. Whereas RhCE, RhD and RhAG are erythroid specific, RhBG and RhCG are expressed in key organs associated with ammonium transport and metabolism. We have investigated the ammonium transport function of human RhBG and RhCG by comparing intracellular pH variation in wild-type and transfected HEK-293 (human embryonic kidney) cells and MDCK (Madin-Darby canine kidney) cells in the presence of ammonium (NH4+/NH3) gradients. Stopped-flow spectrofluorimetry analysis, using BCECF [2',7'-bis-(2-carboxyethyl)-5(6)-carboxyfluorescein] as a pH-sensitive probe, revealed that all cells submitted to inwardly or outwardly directed ammonium gradients exhibited rapid alkalinization or acidification phases respectively, which account for ammonium movements in transfected and native cells. However, as compared with wild-type cells known to have high NH3 lipid permeability, RhBG- and RhCG-expressing cells exhibited ammonium transport characterized by: (i) a five to six times greater kinetic rate-constant; (ii) a weak temperature-dependence; and (iii) reversible inhibition by mercuric chloride (IC50: 52 microM). Similarly, when subjected to a methylammonium gradient, RhBG- and RhCG-expressing cells exhibited kinetic rate constants greater than those of native cells. However, these constants were five times higher for RhBG as compared with RhCG, suggesting a difference in substrate accessibility. These results, indicating that RhBG and RhCG facilitate rapid and low-energy-dependent bi-directional ammonium movement across the plasma membrane, favour the hypothesis that these Rh glycoproteins, together with their erythroid homologue RhAG [Ripoche, Bertrand, Gane, Birkenmeier, Colin and Cartron (2005) Proc. Natl. Acad. Sci. U.S.A. 101, 17222-17227] constitute a family of NH3 channels in mammalian cells.
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