ArticleThe EMBO journal1985
Identification of multiple tubulins in taxol microtubules purified from carrot suspension cells.
Article in The EMBO journal, 1985. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed, 35 citations in OpenAlex.
- Changes in the accumulation of alpha- and beta-tubulin during bud development in Vitis vinifera L.Planta · 2010Article
- Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.Planta · 1997Article
- Tubulin Isotypes in Rye Roots Are Altered during Cold Acclimation.Plant physiology · 1990Article
- cDNA cloning of β-tubulin gene and organization of tubulin genes in Vigna radiata (mung bean) genome.Plant molecular biology · 1987Article
- The relatively large beta-tubulin gene family of Arabidopsis contains a member with an unusual transcribed 5' noncoding sequence.Plant molecular biology · 1987Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tubulin has been purified from carrot suspension cells by ion-exchange chromatography and assembled into microtubules in the presence of 20 microM taxol. One-dimensional SDS-PAGE suggested that the alpha band migrated faster than the beta band (as has been established for some lower eukaryotic tubulins) and this heterology with brain tubulins was confirmed by peptide mapping. When subjected to two-dimensional gel electrophoresis, the plant tubulins could be separated into multiple alpha and beta isotypes. Immunoblotting, using monoclonal anti-tubulins, confirmed that the tubulin isotypes identified in taxol microtubules represent all of the tubulins present in homogenates of unsynchronised log-phase carrot suspension cells. All identified tubulins are therefore assembly-competent under these conditions. Plant cells can contain four different microtubule arrays, but cells arrested in G0/G1 contain only cortical microtubule arrays; such cells, however, exhibit the same tubulin profile as non-synchronised cells, thereby showing no restriction in the number of subunits during this phase of the cell cycle.
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Registered trials
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