ArticleJournal of biomolecular NMR2005
Residual backbone and side-chain 13C and 15N resonance assignments of the intrinsic transmembrane light-harvesting 2 protein complex by solid-state Magic Angle Spinning NMR spectroscopy.
Article in Journal of biomolecular NMR, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 48 citations in OpenAlex.
- Insights into the photoprotective switch of the major light-harvesting complex II (LHCII): a preserved core of arginine-glutamate interlocked helices complemented by adjustable loops.The Journal of biological chemistry · 2013Article
- Solid-state NMR applied to photosynthetic light-harvesting complexes.Photosynthesis research · 2012Review
- A rapid and robust method for selective isotope labeling of proteins.Methods (San Diego, Calif.) · 2011Article
- Atomic resolution protein structure determination by three-dimensional transferred echo double resonance solid-state nuclear magnetic resonance spectroscopy.The Journal of chemical physics · 2009Article
- Assigning large proteins in the solid state: a MAS NMR resonance assignment strategy using selectively and extensively 13C-labelled proteins.Journal of biomolecular NMR · 2009Article
- Advances towards resonance assignments for uniformly--13C, 15N enriched bacteriorhodopsin at 18.8 T in purple membranes.Journal of biomolecular NMR · 2008Article
- Solid-state NMR spectroscopy of human immunodeficiency virus fusion peptides associated with host-cell-like membranes: 2D correlation spectra and distance measurements support a fully extended conformation and models for specific antiparallel strand registries.Journal of the American Chemical Society · 2008Article
- Chemical shift assignment of the transmembrane helices of DsbB, a 20-kDa integral membrane enzyme, by 3D magic-angle spinning NMR spectroscopy.Protein science : a publication of the Protein Society · 2008Article
- Magic-angle spinning solid-state NMR spectroscopy of nanodisc-embedded human CYP3A4.Biochemistry · 2007Article
- Combined solid state and solution NMR studies of alpha,epsilon-15N labeled bovine rhodopsin.Journal of biomolecular NMR · 2007Article
- Signal assignment and secondary structure analysis of a uniformly [13C, 15N]-labeled membrane protein, H +-ATP synthase subunit c, by magic-angle spinning solid-state NMR.Journal of biomolecular NMR · 2006Article
- Structural analysis of nanoscale self-assembled discoidal lipid bilayers by solid-state NMR spectroscopy.Biophysical journal · 2006Article
- Magic-angle spinning solid-state NMR of a 144 kDa membrane protein complex: E. coli cytochrome bo3 oxidase.Journal of biomolecular NMR · 2006Article
- Structure of tightly membrane-bound mastoparan-X, a G-protein-activating peptide, determined by solid-state NMR.Biophysical journal · 2006Article
- Solid-state NMR spectroscopy of 10% 13C labeled ubiquitin: spectral simplification and stereospecific assignment of isopropyl groups.Journal of biomolecular NMR · 2006Article
- Frequency-selective homonuclear dipolar recoupling in solid state NMR.The Journal of chemical physics · 2006Article
- 13C, 15N resonance assignment of parts of the HET-s prion protein in its amyloid form.Journal of biomolecular NMR · 2006Article
- Reconstitution and alignment by a magnetic field of a beta-barrel membrane protein in bicelles.European biophysics journal : EBJ · 2006Article
- Magic Angle Spinning (MAS) NMR: a new tool to study the spatial and electronic structure of photosynthetic complexes.Photosynthesis researchReview
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study reports the sequence specific chemical shifts assignments for 76 residues of the 94 residues containing monomeric unit of the photosynthetic light-harvesting 2 transmembrane protein complex from Rhodopseudomonas acidophila strain 10050, using Magic Angle Spinning (MAS) NMR in combination with extensive and selective biosynthetic isotope labeling methods. The sequence specific chemical shifts assignment is an essential step for structure determination by MAS NMR. Assignments have been performed on the basis of 2-dimensional proton-driven spin diffusion (13)C-(13)C correlation experiments with mixing times of 20 and 500 ms and band selective (13)C-(15)N correlation spectroscopy on a series of site-specific biosynthetically labeled samples. The decreased line width and the reduced number of correlation signals of the selectively labeled samples with respect to the uniformly labeled samples enable to resolve the narrowly distributed correlation signals of the backbone carbons and nitrogens involved in the long alpha-helical transmembrane segments. Inter-space correlations between nearby residues and between residues and the labeled BChl a cofactors, provided by the (13)C-(13)C correlation experiments using a 500 ms spin diffusion period, are used to arrive at sequence specific chemical shift assignments for many residues in the protein complex. In this way it is demonstrated that MAS NMR methods combined with site-specific biosynthetic isotope labeling can be used for sequence specific assignment of the NMR response of transmembrane proteins.
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