ArticleProceedings of the National Academy of Sciences of the United States of America2005
Building a human kinase gene repository: bioinformatics, molecular cloning, and functional validation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- A Novel Signaling Complex between TROY and EGFR Mediates Glioblastoma Cell Invasion.Molecular cancer research : MCR · 2018Article
- Controllability analysis of the directed human protein interaction network identifies disease genes and drug targets.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Design and Structural Characterization of Potent and Selective Inhibitors of Phosphatidylinositol 4 Kinase IIIβ.Journal of medicinal chemistry · 2016Article
- Sustainability in a Hospital-Based Biobank and University-Based DNA Biorepository: Strategic Roadmaps.Biopreservation and biobanking · 2015Article
- Oncogenic Signaling Adaptor Proteins.Journal of genetics and genomics = Yi chuan xue bao · 2015Review
- GLAD: an Online Database of Gene List Annotation for Drosophila.Journal of genomics · 2015Article
- Global analysis of human nonreceptor tyrosine kinase specificity using high-density peptide microarrays.Journal of proteome research · 2014Article
- Exploration of panviral proteome: high-throughput cloning and functional implications in virus-host interactions.Theranostics · 2014Article
- The sedoheptulose kinase CARKL directs macrophage polarization through control of glucose metabolism.Cell metabolism · 2012Article
- PSI:Biology-materials repository: a biologist's resource for protein expression plasmids.Journal of structural and functional genomics · 2011Article
- Towards systematic functional characterization of cancer genomes.Nature reviews. Genetics · 2011Review
- Human SBK1 is dysregulated in multiple cancers and promotes survival of ovary cancer SK-OV-3 cells.Molecular biology reports · 2011Article
- High-throughput ectopic expression screen for tamoxifen resistance identifies an atypical kinase that blocks autophagy.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Nucleic acid programmable protein array a just-in-time multiplexed protein expression and purification platform.Methods in enzymology · 2011Article
- Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage.Journal of proteome research · 2010Article
- High-throughput screens in diploid cells identify factors that contribute to the acquisition of chromosomal instability.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Analysis of serine-threonine kinase specificity using arrayed positional scanning peptide libraries.Current protocols in molecular biology · 2010Article
- Generation and validation of a Shewanella oneidensis MR-1 clone set for protein expression and phage display.PloS one · 2008Article
- Production and sequence validation of a complete full length ORF collection for the pathogenic bacterium Vibrio cholerae.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- A biomedically enriched collection of 7000 human ORF clones.PloS one · 2008Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinases catalyze the phosphorylation of proteins, lipids, sugars, nucleosides, and other important cellular metabolites and play key regulatory roles in all aspects of eukaryotic cell physiology. Here, we describe the mining of public databases to collect the sequence information of all identified human kinase genes and the cloning of the corresponding ORFs. We identified 663 genes, 511 encoding protein kinases, and 152 encoding nonprotein kinases. We describe the successful cloning and sequence verification of 270 of these genes. Subcloning of this gene set in mammalian expression vectors and their use in high-throughput cell-based screens allowed the validation of the clones at the level of expression and the identification of previously uncharacterized modulators of the survivin promoter. Moreover, expressions of the kinase genes in bacteria, followed by autophosphorylation assays, identified 21 protein kinases that showed autocatalytic activity. The work described here will facilitate the functional assaying of this important gene family in phenotypic screens and their use in biochemical and structural studies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.