ArticleIn vitro cellular & developmental biology. Animal
Leukemia inhibitory factor as an anti-apoptotic mitogen for pluripotent mouse embryonic stem cells in a serum-free medium without feeder cells.
Article in In vitro cellular & developmental biology. Animal. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 75 citations in OpenAlex.
- Leukemia inhibitory factor promotes growth and maintains stemness in giant panda MSCs.In vitro cellular & developmental biology. Animal · 2025Article
- The protective effect of leukemia inhibitory factor on apoptosis of BMSCs induced by hypoxia and serum-deprivation.American journal of translational research · 2023Article
- Molecular Mechanisms of RSV and Air Pollution Interaction: A Scoping Review.International journal of molecular sciences · 2022Article
- Thyroid hormone enhances stem cell maintenance and promotes lineage-specific differentiation in human embryonic stem cells.Stem cell research & therapy · 2022Article
- Cigarette smoke exposure reduces leukemia inhibitory factor levels during respiratory syncytial viral infection.International journal of chronic obstructive pulmonary disease · 2019Article
- Asymmetricity Between Sister Cells of Pluripotent Stem Cells at the Onset of Differentiation.Stem cells and development · 2018Article
- Leukemia inhibitory factor increases the proliferation of human endometrial stromal cells and expression of genes related to pluripotency.International journal of reproductive biomedicine · 2017Article
- Nik-related kinase regulates trophoblast proliferation and placental development by modulating AKT phosphorylation.PloS one · 2017Article
- Biological Effects of Culture Substrates on Human Pluripotent Stem Cells.Stem cells international · 2016Review
- Synergistic effects of FGF-2 and Activin A on early neural differentiation of human pluripotent stem cells.In vitro cellular & developmental biology. Animal · 2015Article
- Low-molecular-weight inhibitors of cell differentiation enable efficient growth of mouse iPS cells under feeder-free conditions.Cytotechnology · 2015Article
- Improved efficiency of definitive endoderm induction from human induced pluripotent stem cells in feeder and serum-free culture system.In vitro cellular & developmental biology. Animal · 2015Article
- A miR-590/Acvr2a/Rad51b axis regulates DNA damage repair during mESC proliferation.Stem cell reports · 2014Article
- Leukemia inhibitory factor protects the lung during respiratory syncytial viral infection.BMC immunology · 2014Article
- Protein kinase C-induced early growth response protein-1 binding to SNAIL promoter in epithelial-mesenchymal transition of human embryonic stem cells.Stem cells and development · 2014Article
- Derivation and maintenance of murine trophoblast stem cells under defined conditions.Stem cell reports · 2014Article
- Immortalized tumor derived rat fibroblasts as feeder cells facilitate the cultivation of male embryonic stem cells from the rat strain WKY/Ztm.SpringerPlus · 2014Article
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- Effect of small molecule supplements during in vitro culture of mouse zygotes and parthenogenetic embryos on hypoblast formation and stem cell derivation.Stem cell reviews and reports · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 6 institutions in 2 countries.
Funding
Abstract
We have developed a serum-free medium, designated ESF7, in which leukemia inhibitory factor (LIF) clearly stimulated murine embryonic stem (ES) cell proliferation accompanied by increased expression of nanog and Rex-1 and decreased FGF-5 expression. These effects were dependent on the concentration of LIF. The ES cells maintained in ESF7 medium for more than 2 yr retained an undifferentiated phenotype, as manifested by the expression of the transcription factor Oct-3/4, the stem cell marker SSEA-1, and alkaline phosphatase. Withdrawal of LIF from ESF7 medium resulted in ES cell apoptosis. Addition of serum to ESF7 medium promoted ES cell differentiation. Addition of BMP4 promoted ES cell differentiation into simple epithelial-like cells. In contrast, FGF-2 promoted ES cell differentiation into neuronal and glial-like cells. Under serum-free culture conditions, LIF was sufficient to stimulate cell proliferation, it inhibited cell differentiation, and it maintained self-renewal of ES cells. Because this simple serum-free adherent monoculture system supports the long-term propagation of pluripotent ES cells in vitro, it will allow the elucidation of ES cell responses to growth factors under defined conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.