ArticleMolecular and cellular biology2005
Reduced genomic cytosine methylation and defective cellular differentiation in embryonic stem cells lacking CpG binding protein.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
39 citing papers in PubMed, 98 citations in OpenAlex.
- Temporal analysis of two inducible human genes reveals post-transcriptional H3K4me3 deposition.Life science alliance · 2026Article
- CXXC Finger Protein 1 drives BMP signaling and progenitor cell differentiation during limb development.Developmental biology · 2025Article
- CFP1 governs uterine epigenetic landscapes to intervene in progesterone responses for uterine physiology and suppression of endometriosis.Nature communications · 2023Article
- Epigenetic regulator Cfp1 safeguards male meiotic progression by regulating meiotic gene expression.Experimental & molecular medicine · 2022Article
- Uncoupling histone H3K4 trimethylation from developmental gene expression via an equilibrium of COMPASS, Polycomb and DNA methylation.Nature genetics · 2020Article
- Hansel, Gretel, and the Consequences of Failing to Remove Histone Methylation Breadcrumbs.Trends in genetics : TIG · 2020Review
- CpG binding protein (CFP1) occupies open chromatin regions of active genes, including enhancers and non-CpG islands.Epigenetics & chromatin · 2018Article
- DNA methylation regulates discrimination of enhancers from promoters through a H3K4me1-H3K4me3 seesaw mechanism.BMC genomics · 2017Article
- The SET1 Complex Selects Actively Transcribed Target Genes via Multivalent Interaction with CpG Island Chromatin.Cell reports · 2017Article
- DUSP9 Modulates DNA Hypomethylation in Female Mouse Pluripotent Stem Cells.Cell stem cell · 2017Article
- SET/MLL family proteins in hematopoiesis and leukemia.International journal of hematology · 2017Review
- Article
- Computational inference of a genomic pluripotency signature in human and mouse stem cells.Biology direct · 2016Article
- CXXC finger protein 1 is critical for T-cell intrathymic development through regulating H3K4 trimethylation.Nature communications · 2016Article
- Article
- Cfp1 is required for gene expression-dependent H3K4 trimethylation and H3K9 acetylation in embryonic stem cells.Genome biology · 2014Article
- The epigenetic regulator CXXC finger protein 1 is essential for murine hematopoiesis.PloS one · 2014Article
- Discovery of MLL1 binding units, their localization to CpG Islands, and their potential function in mitotic chromatin.BMC genomics · 2013Article
- MMDiff: quantitative testing for shape changes in ChIP-Seq data sets.BMC genomics · 2013Article
- Histone H3 lysine 4 methyltransferases and demethylases in self-renewal and differentiation of stem cells.Cell & bioscience · 2013Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Cytosine methylation at CpG dinucleotides is a critical epigenetic modification of mammalian genomes. CpG binding protein (CGBP) exhibits a unique DNA-binding specificity for unmethylated CpG motifs and is essential for early murine development. Embryonic stem cell lines deficient for CGBP were generated to further examine CGBP function. CGBP(-)(/)(-) cells are viable but show an increased rate of apoptosis and are unable to achieve in vitro differentiation following removal of leukemia inhibitory factor from the growth media. Instead, CGBP(-)(/)(-) embryonic stem cells remain undifferentiated as revealed by persistent expression of the pluripotent markers Oct4 and alkaline phosphatase. CGBP(-)(/)(-) cells exhibit a 60 to 80% decrease in global cytosine methylation, including hypo-methylation of repetitive elements, single-copy genes, and imprinted genes. Total DNA methyltransferase activity is reduced by 30 to 60% in CGBP(-)(/)(-) cells, and expression of the maintenance DNA methyltransferase 1 protein is similarly reduced. However, de novo DNA methyltransferase activity is normal. Nearly all aspects of the pleiotropic CGBP(-)(/)(-) phenotype are rescued by introduction of a CGBP expression vector. Hence, CGBP is essential for normal epigenetic modification of the genome by cytosine methylation and for cellular differentiation, consistent with the requirement for CGBP during early mammalian development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.