ArticleThe British journal of ophthalmology2005
The use of human serum in supporting the in vitro and in vivo proliferation of human conjunctival epithelial cells.
Article in The British journal of ophthalmology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Global transcriptional analysis of human FHs 74 Int intestinal epithelial cells after exposure to advanced glycation end products.PloS one · 2025Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Autologous plasma versus fetal calf serum as a supplement for the culture of neutrophils.BMC research notes · 2020Article
- Conjunctival goblet cells: Ocular surface functions, disorders that affect them, and the potential for their regeneration.The ocular surface · 2020Review
- Interleukin-13 maintains the stemness of conjunctival epithelial cell cultures prepared from human limbal explants.PloS one · 2019Article
- Article
- Comparison of Human Dermal Fibroblasts and HaCat Cells Cultured in Medium with or without Serum via a Generic Tissue Engineering Research Platform.International journal of molecular sciences · 2018Article
- Artificial Polymeric Scaffolds as Extracellular Matrix Substitutes for Autologous Conjunctival Goblet Cell Expansion.Investigative ophthalmology & visual science · 2016Article
- Macromolecularly crowded in vitro microenvironments accelerate the production of extracellular matrix-rich supramolecular assemblies.Scientific reports · 2015Article
- Effect of growth factors on the proliferation and gene expression of human meibomian gland epithelial cells.Investigative ophthalmology & visual science · 2013Article
- Regeneration of the corneal epithelium with conjunctival epithelial equivalents generated in serum- and feeder-cell-free media.Molecular vision · 2013Article
- Effect of different artificial tears against desiccation in cultured human epithelial cells.Medical science monitor : international medical journal of experimental and clinical research · 2012Article
- Sequential use of human-derived medium supplements favours cardiovascular tissue engineering.Journal of cellular and molecular medicine · 2012Article
- Epithelial microfilament regulators show regional distribution in mouse conjunctiva.Molecular vision · 2010Article
- Cultured human ocular surface epithelium on therapeutic contact lenses.The British journal of ophthalmology · 2007Article
- Plasma polymer coated surfaces for serum-free culture of limbal epithelium for ocular surface disease.Journal of materials science. Materials in medicine · 2007Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo evaluate the use of human serum (HS) in supporting the in vitro and in vivo proliferation of human conjunctival epithelial cells, and compare it with fetal bovine serum (FBS) and bovine pituitary extract (BPE).
methodsConjunctival epithelial cells were cultivated in media supplemented with HS (5%, 10%), FBS (5%, 10%), and BPE (70 microg/ml, 140 microg/ml). The colony forming efficiency (CFE), bromodeoxyuridine (BrdU) ELISA proliferation assay, and cell generations were analysed. Cells were evaluated for keratin (K4, K19, and K3) and MUC5AC expression by immunostaining and RT-PCR. Conjunctival equivalents constructed on amniotic membranes were transplanted onto severe combined immune deficient (SCID) mice for 10 days and analysed histologically.
resultsThe proliferation assays of HS supplemented cultures (CFE, 6.7% (SD 1.8%); BrdU absorbance, 0.86 (0.16)) were comparable to FBS supplemented (CFE, 9.3% (1.8%); BrdU absorbance, 1.11 (0.18)) and BPE supplemented cultures (CFE, 5.9 (1.5); BrdU absorbance, 0.65 (0.12)). Goblet cell densities for HS, FBS, and BPE supplemented media were 52 cells/cm(2), 60 cells/cm(2), and 50 cells/cm(2), respectively. HS supplemented cultures formed stratified epithelial sheets in vivo following transplantation.
conclusionsThe proliferative capacity of conjunctival epithelial cells cultivated in HS supplemented cultures was comparable to FBS and BPE supplemented cultures. The elimination of animal material from the culture system is advantageous when cultivating cells for clinical transplantation.
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