ArticleBiophysical journal2005
Interactions of cholesterol with lipid membranes and cyclodextrin characterized by calorimetry.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Does Complexation of Plasma Membrane Cholesterol with Phospholipids Determine its Transbilayer Distribution?The Journal of membrane biology · 2026Article
- Reversible tuning of membrane sterol levels by cyclodextrin in a dialysis setting.Biophysical journal · 2025Article
- Chemical potential measurements constrain models of cholesterol-phosphatidylcholine interactions.Biophysical journal · 2023Article
- A Guide to Your Desired Lipid-Asymmetric Vesicles.Membranes · 2023Review
- Synergistic Effect of Acetazolamide-(2-hydroxy)propyl β-Cyclodextrin in Timolol Liposomes for Decreasing and Prolonging Intraocular Pressure Levels.Pharmaceutics · 2021Article
- The Lord of the NanoRings: Cyclodextrins and the battle against SARS-CoV-2.International journal of pharmaceutics · 2020Review
- Stairway to Asymmetry: Five Steps to Lipid-Asymmetric Proteoliposomes.Biophysical journal · 2020Article
- Cholesterol-Induced Conformational Change in the Sphingomyelin Headgroup.Biophysical journal · 2019Article
- The Affinity of Sterols for Different Phospholipid Classes and Its Impact on Lateral Segregation.Biophysical journal · 2019Article
- Preparation of Asymmetric Liposomes Using a Phosphatidylserine Decarboxylase.Biophysical journal · 2018Article
- The Chemical Potential of Plasma Membrane Cholesterol: Implications for Cell Biology.Biophysical journal · 2018Article
- Effect of Lung Surfactant Protein SP-C and SP-C-Promoted Membrane Fragmentation on Cholesterol Dynamics.Biophysical journal · 2016Article
- The Affinity of Cholesterol for Different Phospholipids Affects Lateral Segregation in Bilayers.Biophysical journal · 2016Article
- Cholesterol Depletion from a Ceramide/Cholesterol Mixed Monolayer: A Brewster Angle Microscope Study.Scientific reports · 2016Article
- Depletion with Cyclodextrin Reveals Two Populations of Cholesterol in Model Lipid Membranes.Biophysical journal · 2016Article
- Effect of Cyclodextrins on Morphology and Barrier Characteristics of Isolated Rabbit Corneas.AAPS PharmSciTech · 2015Article
- Article
- Stability and stoichiometry of bilayer phospholipid-cholesterol complexes: relationship to cellular sterol distribution and homeostasis.Biochemistry · 2013Article
- An electron paramagnetic resonance method for measuring the affinity of a spin-labeled analog of cholesterol for phospholipids.The Journal of membrane biology · 2013Article
- Computational microscopy of cyclodextrin mediated cholesterol extraction from lipid model membranes.Scientific reports · 2013Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interactions of cholesterol (cho) with different lipids are commonly believed to play a key role in the formation of functional domains in membranes. We introduce a novel approach to characterize cho-lipid interactions by isothermal titration calorimetry. Cho is solubilized in the aqueous phase by reversible complexation with methyl-beta-cyclodextrin (cyd). Uptake of cho into the membrane is measured upon a series of injections of lipid vesicles into a cyd/cho solution. As an independent assay, cho release from membranes is measured upon titrating lipid/cho mixed vesicles into a cyd solution. The most consistent fit to the data is obtained with a mole fraction (rather than mole ratio) partition coefficient and considering a cho/cyd stoichiometry of 1:2. The results are discussed in terms of contributions from 1), the transfer of cho from cyd into a hypothetical, ideally mixed membrane and 2), from nonideal interactions with POPC. The latter are exothermic but opposed by a strong loss in entropy, in agreement with cho-induced acyl chain ordering and membrane condensation. They are accompanied by a positive heat capacity change which cannot be interpreted in terms of the hydrophobic effect, suggesting that additive-induced chain ordering itself increases the heat capacity. The new assays have a great potential for a better understanding of sterol-lipid interactions and yield suggestions how to optimize cho extraction from membranes.
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