ArticleBiophysical journal2005
Accuracy and dynamic range of spatial image correlation and cross-correlation spectroscopy.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 81 citations in OpenAlex.
- Measurement of Protein Transport in Heterogeneous Environments Using Confinement k-Space Image Correlation Spectroscopy.Biomolecules · 2026Article
- Polarized Phase-Sensitive Fluorescence-Image Correlation Spectroscopy.Biomolecules · 2026Article
- ESPRESSO: spatiotemporal omics based on organelle phenotyping.Nature methods · 2025Article
- Quantifying superimposed protein flow dynamics in live cells using spatial filtering and spatiotemporal image correlation spectroscopy.Journal of microscopy · 2025Article
- Phase-Sensitive Fluorescence Image Correlation Spectroscopy.International journal of molecular sciences · 2024Article
- The LDL receptor is regulated by membrane cholesterol as revealed by fluorescence fluctuation analysis.Biophysical journal · 2023Article
- The coordination of spindle-positioning forces during the asymmetric division of the Caenorhabditis elegans zygote.EMBO reports · 2021Article
- Microtubule Feedback and LET-99-Dependent Control of Pulling Forces Ensure Robust Spindle Position.Biophysical journal · 2018Article
- Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy.Journal of visualized experiments : JoVE · 2018Article
- Probing the intracellular refractive index and molecular interaction of gold nanoparticles in HeLa cells using single particle spectroscopy.International journal of nanomedicine · 2018Article
- Dense transcript profiling in single cells by image correlation decoding.Nature methods · 2016Article
- Netrin-1-Regulated Distribution of UNC5B and DCC in Live Cells Revealed by TICCS.Biophysical journal · 2016Article
- An Intermittent Model for Intracellular Motions of Gold Nanostars by k-Space Scattering Image Correlation.Biophysical journal · 2015Article
- New automated single-cell technique for segmentation and quantitation of lipid droplets.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Recruitment of the adaptor protein Grb2 to EGFR tetramers.Biochemistry · 2014Article
- Dynamic transition states of ErbB1 phosphorylation predicted by spatial stochastic modeling.Biophysical journal · 2013Article
- A nu-space for ICS: characterization and application to measure protein transport in live cells.New journal of physics · 2013Article
- Aggregation distributions on cells determined by photobleaching image correlation spectroscopy.Biophysical journal · 2013Article
- Fluorescence fluctuation approaches to the study of adhesion and signaling.Methods in enzymology · 2013Article
- Theory and practical recommendations for autocorrelation-based image correlation spectroscopy.Journal of biomedical optics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present a comprehensive study of the accuracy and dynamic range of spatial image correlation spectroscopy (ICS) and image cross-correlation spectroscopy (ICCS). We use simulations to model laser scanning microscopy imaging of static subdiffraction limit fluorescent proteins or protein clusters in a cell membrane. The simulation programs allow us to control the spatial imaging sampling variables and the particle population densities and interactions and introduce and vary background and counting noise typical of what is encountered in digital optical microscopy. We systematically calculate how the accuracy of both image correlation methods depends on practical experimental collection parameters and characteristics of the sample. The results of this study provide a guide to appropriately plan spatial image correlation measurements on proteins in biological membranes in real cells. The data presented map regimes where the spatial ICS and ICCS provide accurate results as well as clearly showing the conditions where they systematically deviate from acceptable accuracy. Finally, we compare the simulated data with standard confocal microscopy using live CHO cells expressing the epidermal growth factor receptor fused with green fluorescent protein (GFP/EGFR) to obtain typical values for the experimental variables that were investigated in our study. We used our simulation results to estimate a relative precision of 20% for the ICS measured receptor density of 64 microm(-2) within a 121 x 98 pixel subregion of a single cell.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.