ArticleBiophysical journal2005
Spatial analysis of 3' phosphoinositide signaling in living fibroblasts, III: influence of cell morphology and morphological Polarity.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Quantitative investigation of MDA-MB-231 breast cancer cell motility: dependence on epidermal growth factor concentration and its gradient.Molecular bioSystems · 2017Article
- Are Filopodia Privileged Signaling Structures in Migrating Cells?Biophysical journal · 2016Article
- F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling.The Journal of cell biology · 2015Article
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- The role of diatom nanostructures in biasing diffusion to improve uptake in a patchy nutrient environment.PloS one · 2013Article
- Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism.The Journal of cell biology · 2012Article
- Investigating circular dorsal ruffles through varying substrate stiffness and mathematical modeling.Biophysical journal · 2011Article
- A comparison of mathematical models for polarization of single eukaryotic cells in response to guided cues.PLoS computational biology · 2011Review
- Computational analysis of the spatiotemporal coordination of polarized PI3K and Rac1 activities in micro-patterned live cells.PloS one · 2011Article
- Directional persistence of cell migration coincides with stability of asymmetric intracellular signaling.Biophysical journal · 2010Article
- SIMULATING BIOCHEMICAL SIGNALING NETWORKS IN COMPLEX MOVING GEOMETRIES.SIAM journal on scientific computing : a publication of the Society for Industrial and Applied Mathematics · 2010Article
- Spontaneous phosphoinositide 3-kinase signaling dynamics drive spreading and random migration of fibroblasts.Journal of cell science · 2009Article
- Article
- Membrane-binding/modification model of signaling protein activation and analysis of its control by cell morphology.Biophysical journal · 2007Article
- Key role of local regulation in chemosensing revealed by a new molecular interaction-based modeling method.PLoS computational biology · 2006Article
- Deterministic model of dermal wound invasion incorporating receptor-mediated signal transduction and spatial gradient sensing.Biophysical journal · 2006Article
- Quantitative elucidation of a distinct spatial gradient-sensing mechanism in fibroblasts.The Journal of cell biology · 2005Article
- Cell population-based model of dermal wound invasion with heterogeneous intracellular signaling properties.Cell adhesion & migrationArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activation of phosphoinositide (PI) 3-kinase is a required signaling pathway in fibroblast migration directed by platelet-derived growth factor. The pattern of 3' PI lipids in the plasma membrane, integrating local PI 3-kinase activity as well as 3' PI diffusion and turnover, influences the spatiotemporal regulation of the cytoskeleton. In fibroblasts stimulated uniformly with platelet-derived growth factor, visualized using total internal reflection fluorescence microscopy, we consistently observed localized regions with significantly higher or lower 3' PI levels than adjacent regions (hot and cold spots, respectively). A typical cell contained multiple hot spots, coinciding with apparent leading edge structures, and at most one cold spot at the rear. Using a framework for finite-element modeling with actual cell contact area geometries, we find that although the 3' PI pattern is affected by irregular contact area shape, cell morphology alone cannot explain the presence of hot or cold spots. Our results and analysis instead suggest that these regions reflect different local 3' PI dynamics, specifically through a combination of mechanisms: enhanced PI 3-kinase activity, reduced 3' PI turnover, and possibly slow/constrained 3' PI diffusion. The morphological polarity of the cell may thus bias 3' PI signaling to promote persistent migration in fibroblasts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.