ArticleMarine pollution bulletin2005
An electrochemical RNA hybridization assay for detection of the fecal indicator bacterium Escherichia coli.
Article in Marine pollution bulletin, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 51 citations in OpenAlex.
- Application of Aptamer-Based Biosensor for Rapid Detection of PathogenicSensors (Basel, Switzerland) · 2018Review
- Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application.Sensors (Basel, Switzerland) · 2016Review
- Magnetic Beads-Based Electrochemical Sensors Applied to the Detection and Quantification of Bioterrorism/Biohazard Agents.Electroanalysis · 2012Review
- New trends in impedimetric biosensors for the detection of foodborne pathogenic bacteria.Sensors (Basel, Switzerland) · 2012Review
- Highly sensitive disposable nucleic acid biosensors for direct bioelectronic detection in raw biological samples.Talanta · 2011Article
- Optimization of two immunofluorescent antibodies for the detection of Escherichia coli using immunofluorescent microscopy and flow cytometry.Current microbiology · 2011Article
- Ternary surface monolayers for ultrasensitive (zeptomole) amperometric detection of nucleic acid hybridization without signal amplification.Analytical chemistry · 2010Article
- Motion-based DNA detection using catalytic nanomotors.Nature communications · 2010Article
- Electrochemical detection of harmful algae and other microbial contaminants in coastal waters using hand-held biosensors.Marine pollution bulletin · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
Monitoring waters for indicator bacteria is required to protect the public from exposure to fecal pollution. Our proof-of-concept study describes a method for detecting fecal coliforms. The coliform Escherichia coli was used as a model fecal indicator. DNA probe-coated magnetic beads in combination with the electrochemical monitoring of the oxidation state of guanine nucleotides should allow for direct detection of bacterial RNA. To demonstrate this concept, we used voltammetry in connection with pencil electrodes to detect isolated E. coli 16S rRNA. Using this approach, 10(7) cells of E. coli were detected in a quantitative, reproducible fashion in 4h. Detection was achieved without a nucleic acid amplification step. The specificity of the assay for coliforms was demonstrated by testing against a panel of bacterial RNA. We also show that E. coli RNA can be detected directly from cell extracts. The method could be used for on-site detection and shows promise for adaptation into automated biosensors for water-quality monitoring.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.