ArticleThe EMBO journal2005
Opposing roles for DNA structure-specific proteins Rad1, Msh2, Msh3, and Sgs1 in yeast gene targeting.
Article in The EMBO journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 32 citations in OpenAlex.
- The Slx4-Rad1-Rad10 nuclease differentially regulates deletions and duplications induced by a replication fork barrier.PLoS genetics · 2025Article
- Gene duplication and deletion caused by over-replication at a fork barrier.Nature communications · 2023Article
- Distinct DNA repair pathways cause genomic instability at alternative DNA structures.Nature communications · 2020Article
- The XPF-ERCC1 Complex Is Essential for Genome Stability and Is Involved in the Mechanism of Gene Targeting inFrontiers in plant science · 2019Article
- Mechanisms of gene targeting in higher eukaryotes.Cellular and molecular life sciences : CMLS · 2016Review
- Sgs1 and Exo1 suppress targeted chromosome duplication during ends-in and ends-out gene targeting.DNA repair · 2014Article
- The Rad1-Rad10 nuclease promotes chromosome translocations between dispersed repeats.Nature structural & molecular biology · 2012Article
- A tale of tails: insights into the coordination of 3' end processing during homologous recombination.BioEssays : news and reviews in molecular, cellular and developmental biology · 2009Review
- Aberrant double-strand break repair resulting in half crossovers in mutants defective for Rad51 or the DNA polymerase delta complex.Molecular and cellular biology · 2009Article
- DNA curtains and nanoscale curtain rods: high-throughput tools for single molecule imaging.Langmuir : the ACS journal of surfaces and colloids · 2008Article
- Sequence divergence impedes crossover more than noncrossover events during mitotic gap repair in yeast.Genetics · 2008Article
- Microarray-based genetic screen defines SAW1, a gene required for Rad1/Rad10-dependent processing of recombination intermediates.Molecular cell · 2008Article
- Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases.Nucleic acids research · 2007Article
- The structure-specific endonuclease Mus81-Eme1 promotes conversion of interstrand DNA crosslinks into double-strands breaks.The EMBO journal · 2006Article
- MSH2 is essential for the preservation of genome integrity and prevents homeologous recombination in the moss Physcomitrella patens.Nucleic acids research · 2006Article
- Parameters determining the efficiency of gene targeting in the moss Physcomitrella patens.Nucleic acids research · 2005Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Targeted gene replacement (TGR) in yeast and mammalian cells is initiated by the two free ends of the linear targeting molecule, which invade their respective homologous sequences in the chromosome, leading to replacement of the targeted locus with a selectable gene from the targeting DNA. To study the postinvasion steps in recombination, we examined the effects of DNA structure-specific proteins on TGR frequency and heteroduplex DNA formation. In strains deleted of RAD1, MSH2, or MSH3, we find that the frequency of TGR is reduced and the mechanism of TGR is altered while the reverse is true for deletion of SGS1, suggesting that Rad1 and Msh2:Msh3 facilitate TGR while Sgs1 opposes it. The altered mechanism of TGR in the absence of Msh2:Msh3 and Rad1 reveals a separate role for these proteins in suppressing an alternate gene replacement pathway in which incorporation of both homology regions from a single strand of targeting DNA into heteroduplex with the targeted locus creates a mismatch between the selectable gene on the targeting DNA and the targeted gene in the chromosome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.