ArticleJournal of virology2005
Proteome analysis of liver cells expressing a full-length hepatitis C virus (HCV) replicon and biopsy specimens of posttransplantation liver from HCV-infected patients.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
38 citing papers in PubMed, 80 citations in OpenAlex.
- Hepatitis C virus treatment with direct-acting antivirals induces rapid changes in the hepatic proteome.Journal of viral hepatitis · 2021Article
- Signalome-wide assessment of host cell response to hepatitis C virus.Nature communications · 2017Article
- Proteomic approaches to analyzing hepatitis C virus biology.Proteomics · 2015Review
- Epitope Prediction Assays Combined with Validation Assays Strongly Narrows down Putative Cytotoxic T Lymphocyte Epitopes.Vaccines · 2015Article
- Quantitative Proteomics Analysis of the Hepatitis C Virus Replicon High-Permissive and Low-Permissive Cell Lines.PloS one · 2015Article
- Curing a viral infection by targeting the host: the example of cyclophilin inhibitors.Antiviral research · 2013Review
- Genome-wide analysis of host mRNA translation during hepatitis C virus infection.Journal of virology · 2013Article
- Affinity capture and identification of host cell factors associated with hepatitis C virus (+) strand subgenomic RNA.Molecular & cellular proteomics : MCP · 2013Article
- Proteome analyses of strains ATCC 51142 and PCC 7822 of the diazotrophic cyanobacterium Cyanothece sp. under culture conditions resulting in enhanced H₂ production.Applied and environmental microbiology · 2013Article
- Proteomic analysis of the effect of fuzheng huayu recipe on fibrotic liver in rats.Evidence-based complementary and alternative medicine : eCAM · 2013Article
- Proteome and computational analyses reveal new insights into the mechanisms of hepatitis C virus-mediated liver disease posttransplantation.Hepatology (Baltimore, Md.) · 2012Article
- Topological analysis of protein co-abundance networks identifies novel host targets important for HCV infection and pathogenesis.BMC systems biology · 2012Article
- Coupled transcriptome and proteome analysis of human lymphotropic tumor viruses: insights on the detection and discovery of viral genes.BMC genomics · 2011Article
- Dynamic proteomic profiling of a unicellular cyanobacterium Cyanothece ATCC51142 across light-dark diurnal cycles.BMC systems biology · 2011Article
- Extracellular matrix bioengineering and systems biology approaches in liver disease.Systems and synthetic biology · 2011Article
- Diurnal rhythms result in significant changes in the cellular protein complement in the cyanobacterium Cyanothece 51142.PloS one · 2011Article
- Clinical proteomics for liver disease: a promising approach for discovery of novel biomarkers.Proteome science · 2010Article
- Macaque proteome response to highly pathogenic avian influenza and 1918 reassortant influenza virus infections.Journal of virology · 2010Article
- Transcriptome sequencing, microarray, and proteomic analyses reveal cellular and metabolic impact of hepatitis C virus infection in vitro.Hepatology (Baltimore, Md.) · 2010Article
- Activity-based protein profiling of the hepatitis C virus replication in Huh-7 hepatoma cells using a non-directed active site probe.Proteome science · 2010Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
The development of a reproducible model system for the study of hepatitis C virus (HCV) infection has the potential to significantly enhance the study of virus-host interactions and provide future direction for modeling the pathogenesis of HCV. While there are studies describing global gene expression changes associated with HCV infection, changes in the proteome have not been characterized. We report the first large-scale proteome analysis of the highly permissive Huh-7.5 cell line containing a full-length HCV replicon. We detected >4,200 proteins in this cell line, including HCV replicon proteins, using multidimensional liquid chromatographic (LC) separations coupled to mass spectrometry. Consistent with the literature, a comparison of HCV replicon-positive and -negative Huh-7.5 cells identified expression changes of proteins involved in lipid metabolism. We extended these analyses to liver biopsy material from HCV-infected patients where a total of >1,500 proteins were detected from only 2 mug of liver biopsy protein digest using the Huh-7.5 protein database and the accurate mass and time tag strategy. These findings demonstrate the utility of multidimensional proteome analysis of the HCV replicon model system for assisting in the determination of proteins/pathways affected by HCV infection. Our ability to extend these analyses to the highly complex proteome of small liver biopsies with limiting protein yields offers the unique opportunity to begin evaluating the clinical significance of protein expression changes associated with HCV infection.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.