ArticleAntimicrobial agents and chemotherapy2005
Proline conformation-dependent antimicrobial activity of a proline-rich histone h1 N-terminal Peptide fragment isolated from the skin mucus of Atlantic salmon.
Article in Antimicrobial agents and chemotherapy, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 99 citations in OpenAlex.
- Bactericidal activity of mammalian histones is caused by large membrane pore formation.Cell reports · 2025Article
- Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infections.mSystems · 2024Article
- From inside to outside: exploring extracellular antimicrobial histone-derived peptides as multi-talented molecules.The Journal of antibiotics · 2024Review
- Identification, Synthesis, and In Vitro Activities of Antimicrobial Peptide from African Catfish against the Extended-Spectrum Beta-Lactamase (ESBL)-ProducingPharmaceutics · 2024Article
- Fractionation of the Caspian sand goby epidermal exudates using membrane ultrafiltration and reversed-phase chromatography: an investigation on bioactivities.Scientific reports · 2024Article
- Fish Skin Mucus Extracts: An Underexplored Source of Antimicrobial Agents.Marine drugs · 2023Review
- Fish Epidermal Mucus as a Source of Diverse Therapeutical Compounds.International journal of peptide research and therapeutics · 2023Review
- Histones: The critical players in innate immunity.Frontiers in immunology · 2022Review
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- Article
- Leveraging orthogonal mass spectrometry based strategies for comprehensive sequencing and characterization of ribosomal antimicrobial peptide natural products.Natural product reports · 2021Review
- Identification of Antimicrobial Peptides Isolated From the Skin Mucus of African Catfish,Frontiers in microbiology · 2021Article
- Dietary Inclusion of Black Soldier Fly (Frontiers in immunology · 2021Article
- PepSAVI-MS Reveals a Proline-rich Antimicrobial Peptide inJournal of natural products · 2019Article
- The Farmed Atlantic Salmon (Genes · 2019Article
- Investigations of the Copper Peptide Hepcidin-25 by LC-MS/MS and NMR.International journal of molecular sciences · 2018Article
- Epidermal mucus, a major determinant in fish health: a review.Iranian journal of veterinary research · 2018Review
- Linker Histone in Diseases.International journal of biological sciences · 2017Review
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- Histones as mediators of host defense, inflammation and thrombosis.Future microbiology · 2016Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A 30-residue N-terminally acetylated peptide derived from the N-terminal part of histone H1 was identified as the dominant antimicrobial peptide in skin mucus from Atlantic salmon (Salmo salar). The peptide (termed salmon antimicrobial peptide [SAMP H1]) was purified to homogeneity by a combination of reversed-phase and cation-exchange chromatographies. By Edman degradation of the deacetylated peptide and by sequencing of the PCR-amplified DNA that encodes the peptide, the complete amino acid sequence was determined to be AEVAPAPAAAAPAKAPKKKAAAKPKKAGPS. The theoretical molecular weight of N-terminally acetylated SAMP H1 was calculated to be 2,836, which is the same as that determined by matrix-assisted laser desorption ionization mass spectrometry. The peptide was active against both gram-negative and -positive bacteria. The N-terminal acetyl group was not necessary for activity since deacetylation did not reduce the activity. A synthetic peptide whose sequence was identical to that of the isolated fragment was initially inactive but could be activated by binding it to a cation-exchange column. Treatment of the synthetic peptide when it was bound to the exchange column with peptidylproline cis-trans-isomerase increased the amount of active peptide, indicating that isomerization of the proline peptide bond(s) was necessary for activation of the synthetic peptide. Comparison of the active and inactive forms by circular dichroism and chromatographic analyses suggests that the active form, both the natural and the synthetic forms, is more structured, condensed, and rigid than the inactive form, which has a more nonstructured conformation. This work shows for the first time the importance of proline isomers in the activity of an antimicrobial peptide.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.