ArticleMolecular biology of the cell2005
Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 60 citations in OpenAlex.
- KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation.Journal of translational medicine · 2022Article
- Activated Src requires Cadherin-11, Rac, and gp130 for Stat3 activation and survival of mouse Balb/c3T3 fibroblasts.Cancer gene therapy · 2022Article
- Roads to Stat3 Paved with Cadherins.Cells · 2022Review
- Acetyl-bufalin shows potent efficacy against non-small-cell lung cancer by targeting the CDK9/STAT3 signalling pathway.British journal of cancer · 2021Article
- Effect of caveolin-1 on Stat3-ptyr705 levels in breast and lung carcinoma cells.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2019Article
- PI3k and Stat3: Oncogenes that are Required for Gap Junctional, Intercellular Communication.Cancers · 2019Review
- STAT3 roles in viral infection: antiviral or proviral?Future virology · 2018Review
- A Central Role for STAT3 in Gammaherpesvirus-Life Cycle and -Diseases.Frontiers in microbiology · 2016Review
- Targeted Blockade of JAK/STAT3 Signaling Inhibits Ovarian Carcinoma Growth.Molecular cancer therapeutics · 2015Article
- Screening Active Compounds from Garcinia Species Native to China Reveals Novel Compounds Targeting the STAT/JAK Signaling Pathway.BioMed research international · 2015Article
- A functional assay for gap junctional examination; electroporation of adherent cells on indium-tin oxide.Journal of visualized experiments : JoVE · 2014Article
- Stat3 and gap junctions in normal and lung cancer cells.Cancers · 2014Article
- Review
- Role of STAT3 in cancer metastasis and translational advances.BioMed research international · 2013Review
- Critical analysis of the potential for targeting STAT3 in human malignancy.OncoTargets and therapy · 2013Article
- Article
- Stat3 is a positive regulator of gap junctional intercellular communication in cultured, human lung carcinoma cells.BMC cancer · 2012Article
- STAT3 is activated by JAK2 independent of key oncogenic driver mutations in non-small cell lung carcinoma.PloS one · 2012Article
- The R(h)oads to Stat3: Stat3 activation by the Rho GTPases.Experimental cell research · 2011Review
- Gambogic acid inhibits STAT3 phosphorylation through activation of protein tyrosine phosphatase SHP-1: potential role in proliferation and apoptosis.Cancer prevention research (Philadelphia, Pa.) · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the role of Stat3 (signal transducer and activator of transcription-3) in neoplastic transformation by the Large Tumor antigen of Simian Virus 40 (TAg), murine fibroblasts were rendered deficient in Stat3 activity through expression of a Stat3-specific siRNA or a Cre-loxP recombination system. The results demonstrate that growth rate, formation of foci overgrowing a monolayer of normal cells and colony formation in soft agar were dramatically reduced in Stat3-deficient cells. In addition, TAg expression led to increased Stat3 tyrosine phosphorylation, DNA binding, and transcriptional activity, suggesting that Stat3 is required for TAg-mediated neoplasia. Stat3 activation was prevented by blocking the binding of TAg to pRb (retinoblastoma-susceptibility gene product), whereas genetic ablation of pRb increased Stat3 activity, suggesting that pRb inactivation by TAg might be responsible for the observed Stat3 activation. Stat3 activation by TAg was suppressed after inhibition of c-Src, JAKs or the insulin-like growth factor receptor. On the other hand, targeted disruption of the Fer kinase or pharmacological inhibition of Abl had no effect. Inhibition of Src activity led to Stat3 down-regulation as well as apoptosis of sparsely growing, TAg-transformed cells. However, Src inhibition was relatively ineffective in confluent cells, consistent with previous results indicating that cell to cell adhesion activates Stat3 by a Src-independent mechanism. Direct Stat3 inhibition on the other hand induced apoptosis very effectively in confluent cells, which could have significant therapeutic implications. Taken together, our results suggest that Stat3 is an important component of a pathway emanating from TAg and leading to neoplastic conversion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.