ArticleThe Journal of biological chemistry2005
Association between Archaeal prolyl- and leucyl-tRNA synthetases enhances tRNA(Pro) aminoacylation.
Article in The Journal of biological chemistry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- The tRNA identity landscape for aminoacylation and beyond.Nucleic acids research · 2023Review
- A Leucyl-tRNA Synthetase Urzyme: Authenticity of tRNA Synthetase Catalytic Activities and Promiscuous Phosphorylation of Leucyl-5'AMP.International journal of molecular sciences · 2022Article
- Sub-Cellular Localization and Complex Formation by Aminoacyl-tRNA Synthetases in Cyanobacteria: Evidence for Interaction of Membrane-Anchored ValRS with ATP Synthase.Frontiers in microbiology · 2016Article
- Archaeal aminoacyl-tRNA synthetases interact with the ribosome to recycle tRNAs.Nucleic acids research · 2014Article
- Association of a multi-synthetase complex with translating ribosomes in the archaeon Thermococcus kodakarensis.FEBS letters · 2012Article
- An archaeal tRNA-synthetase complex that enhances aminoacylation under extreme conditions.The Journal of biological chemistry · 2011Article
- A self-referential model for the formation of the genetic code.Theory in biosciences = Theorie in den Biowissenschaften · 2008Article
- Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.FEMS microbiology reviews · 2008Review
- Spontaneous trpY mutants and mutational analysis of the TrpY archaeal transcription regulator.Journal of bacteriology · 2007Article
- An aminoacyl-tRNA synthetase:elongation factor complex for substrate channeling in archaeal translation.Nucleic acids research · 2007Article
- tRNA synthetase: tRNA aminoacylation and beyond.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Aminoacyl-tRNA synthetase-containing complexes have been identified in different eukaryotes, and their existence has also been suggested in some Archaea. To investigate interactions involving aminoacyl-tRNA synthetases in Archaea, we undertook a yeast two-hybrid screen for interactions between Methanothermobacter thermautotrophicus proteins using prolyl-tRNA synthetase (ProRS) as the bait. Interacting proteins identified included components of methanogenesis, protein-modifying factors, and leucyl-tRNA synthetase (LeuRS). The association of ProRS with LeuRS was confirmed in vitro by native gel electrophoresis and size exclusion chromatography. Determination of the steady-state kinetics of tRNA(Pro) charging showed that the catalytic efficiency (k(cat)/K(m)) of ProRS increased 5-fold in the complex with LeuRS compared with the free enzyme, whereas the K(m) for proline was unchanged. No significant changes in the steady-state kinetics of LeuRS aminoacylation were observed upon the addition of ProRS. These findings indicate that ProRS and LeuRS associate in M. thermautotrophicus and suggest that this interaction contributes to translational fidelity by enhancing tRNA aminoacylation by ProRS.
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