Trial reportRespiratory research2005
Interleukin-17A mRNA and protein expression within cells from the human bronchoalveolar space after exposure to organic dust.
Trial report in Respiratory research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Respiratory Diseases Associated With Organic Dust Exposure.The journal of allergy and clinical immunology. In practice · 2024Review
- MyD88 regulates a prolonged adaptation response to environmental dust exposure-induced lung disease.Respiratory research · 2020Article
- Relationship of systemic IL-10 levels with proinflammatory cytokine responsiveness and lung function in agriculture workers.Respiratory research · 2018Article
- The Effect of Inhalant Organic Dust on Bone Health.Current allergy and asthma reports · 2018Review
- A role for B cells in organic dust induced lung inflammation.Respiratory research · 2017Article
- Influence of farming exposure on the development of asthma and asthma-like symptoms.International immunopharmacology · 2014Review
- Exposure to atmospheric particulate matter enhances Th17 polarization through the aryl hydrocarbon receptor.PloS one · 2013Article
- Targeting IL-17 and TH17 cells in chronic inflammation.Nature reviews. Drug discovery · 2012Review
- αβ T cells and a mixed Th1/Th17 response are important in organic dust-induced airway disease.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2012Article
- Farming-associated environmental exposures and effect on atopic diseases.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2012Review
- Respiratory health effects of large animal farming environments.Journal of toxicology and environmental health. Part B, Critical reviews · 2012Review
- Ambient ultrafine particles provide a strong adjuvant effect in the secondary immune response: implication for traffic-related asthma flares.American journal of physiology. Lung cellular and molecular physiology · 2010Article
- Particulate matter-induced airway hyperresponsiveness is lymphocyte dependent.Environmental health perspectives · 2010Article
- Pattern recognition scavenger receptor A/CD204 regulates airway inflammatory homeostasis following organic dust extract exposures.Journal of immunotoxicologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundIn mice, the cytokine interleukin (IL)-17A causes a local accumulation of neutrophils within the bronchoalveolar space. IL-17A may thereby also contribute to an increased local proteolytic burden. In the current study, we determined whether mRNA for IL-17A is elevated and protein expression of IL-17A occurs locally in inflammatory cells within the human bronchoalveolar space during severe inflammation caused by organic dust. We also assessed the expression of the elastinolytic protease MMP-9 in this airway compartment.
methodsSix healthy, non-smoking human volunteers were exposed to organic dust in a swine confinement, a potent stimulus of neutrophil accumulation within the human bronchoalveolar space. Bronchoalveolar lavage (BAL) fluid was harvested 2 weeks before and 24 hours after the exposure and total and differential counts were conducted for inflammatory BAL cells. Messenger RNA for IL-17A was measured using reverse transcript polymerase chain reaction-enzyme linked immunoassay (RT-PCR-ELISA). Intracellular immunoreactivity (IR) for IL-17A and MMP-9, respectively, was determined in BAL cells.
resultsThe exposure to organic dust caused more than a forty-fold increase of mRNA for IL-17A in BAL cells. IL-17A immunoreactivity was detected mainly in BAL lymphocytes, and the number of these IL-17A expressing lymphocytes displayed an eight-fold increase, even though not statistically significant. The increase in IL-17A mRNA was associated with a substantial increase of the number of BAL neutrophils expressing MMP-9 immunoreactivity.
conclusionExposure to organic dust increases local IL-17A mRNA and because there is intracellular expression in BAL lymphocytes, this suggests that IL-17A protein can originate from lymphocytes within the human bronchoalveolar space. The fact that the increased IL-17A mRNA is associated with an increased number of MMP-9-expressing neutrophils is compatible with IL-17A increasing the local proteolytic burden through its neutrophil-accumulating effect.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.