ArticleJournal of the American Society for Mass Spectrometry2005
Determination of affinity constants and response factors of the noncovalent dimer of gramicidin by electrospray ionization mass spectrometry and mathematical modeling.
Article in Journal of the American Society for Mass Spectrometry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
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- Quantifying Biomolecular Interactions Using Slow Mixing Mode (SLOMO) Nanoflow ESI-MS.ACS central science · 2022Article
- Intracellular Protein-Drug Interactions Probed by Direct Mass Spectrometry of Cell Lysates.Angewandte Chemie (International ed. in English) · 2021Article
- Quantification of Protein-Ligand Interactions by Laser Electrospray Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2018Article
- Insight into Signal Response of Protein Ions in Native ESI-MS from the Analysis of Model Mixtures of Covalently Linked Protein Oligomers.Journal of the American Society for Mass Spectrometry · 2017Article
- Quantitation of the Noncovalent Cellular Retinol-Binding Protein, Type 1 Complex Through Native Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2017Article
- Optimization of Electrospray Ionization by Statistical Design of Experiments and Response Surface Methodology: Protein-Ligand Equilibrium Dissociation Constant Determinations.Journal of the American Society for Mass Spectrometry · 2016Article
- SDS-PAGE analysis of Aβ oligomers is disserving research into Alzheimer´s disease: appealing for ESI-IM-MS.Scientific reports · 2015Article
- Reliable determinations of protein-ligand interactions by direct ESI-MS measurements. Are we there yet?Journal of the American Society for Mass Spectrometry · 2012Article
- Compelling advantages of negative ion mode detection in high-mass MALDI-MS for homomeric protein complexes.Journal of the American Society for Mass Spectrometry · 2012Article
- The gramicidin dimer shows both EX1 and EX2 mechanisms of H/D exchange.Journal of the American Society for Mass Spectrometry · 2009Article
- Ultraslow oligomerization equilibria of p53 and its implications.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Identifying nonspecific ligand binding in electrospray ionization mass spectrometry using the reporter molecule method.Journal of the American Society for Mass Spectrometry · 2009Article
- Which electrospray-based ionization method best reflects protein-ligand interactions found in solution? a comparison of ESI, nanoESI, and ESSI for the determination of dissociation constants with mass spectrometry.Journal of the American Society for Mass Spectrometry · 2008Article
- Application of SIMSTEX to oligomerization of insulin analogs and mutants.Journal of the American Society for Mass Spectrometry · 2006Article
- Electrospray mass spectrometry of NeuAc oligomers associated with the C fragment of the tetanus toxin.Journal of the American Society for Mass Spectrometry · 2006Article
- Quantifying Protein-Glycan Interactions Using Native Mass Spectrometry.Mass spectrometry reviewsReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The dimerization of gramicidin, a 15-residue membrane peptide, in solution can be viewed as a model for protein-protein interactions. We reported previously that the dimer can be observed when electrosprayed from organic solvents and that the abundances of the dimer depends on the dielectric constant of the solvent. Here, we report an effort to determine an affinity constant for the dimerization of gramicidin by using gas-phase abundance. Two issues affecting the determination are the electrospray-induced dissociation of the dimer and discrimination in the electrospray of the dimer compared with the monomer. Other methods developed for the purpose of determining affinity from mass spectral abundance do not address the dissociation of the complex in the gas phase or can not be applied for cases of low affinity constant, K(a). We present a mathematical model that uses the ratio of the signal intensities of the dimer and the monomer during a titration. The model also incorporates the dissociation and an electrospray ionization-response factor of the dimer for extracting the affinity constant for the dimerization of gramicidin. The dimerization constants from the new method agree within a factor of two with values reported in the literature.
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