ArticleJournal of the American Society for Mass Spectrometry2005
Evaluation and optimization of electron capture dissociation efficiency in fourier transform ion cyclotron resonance mass spectrometry.
Article in Journal of the American Society for Mass Spectrometry, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Structural characterization of actinomycin D using multiple ion isolation and electron induced dissociation.Journal of the American Society for Mass Spectrometry · 2014Article
- Charge enhancement of single-stranded DNA in negative electrospray ionization using the supercharging reagent meta-nitrobenzyl alcohol.Journal of the American Society for Mass Spectrometry · 2013Article
- Clustered O-glycans of IgA1: defining macro- and microheterogeneity by use of electron capture/transfer dissociation.Molecular & cellular proteomics : MCP · 2010Article
- Periodic sequence distribution of product ion abundances in electron capture dissociation of amphipathic peptides and proteins.Journal of the American Society for Mass Spectrometry · 2009Article
- Combining bottom-up and top-down mass spectrometric strategies for de novo sequencing of the crustacean hyperglycemic hormone from Cancer borealis.Analytical chemistry · 2009Article
- EVALUATION OF THE EXPERIMENTAL PARAMETERS WHICH CONTROL ELECTRON DETACHMENT DISSOCIATION, AND THEIR EFFECT ON THE FRAGMENTATION EFFICIENCY OF GLYCOSAMINOGLYCAN CARBOHYDRATES.International journal of mass spectrometry · 2008Article
- Electron capture dissociation implementation progress in Fourier transform ion cyclotron resonance mass spectrometry.Journal of the American Society for Mass Spectrometry · 2008Article
- Effects of electron kinetic energy and ion-electron inelastic collisions in electron capture dissociation measured using ion nanocalorimetry.Journal of the American Society for Mass Spectrometry · 2008Article
- Characterization of phosphate-containing metabolites by calcium adduction and electron capture dissociation.Journal of the American Society for Mass Spectrometry · 2008Article
- Simultaneous fragmentation of multiple ions using IMS drift time dependent collision energies.Journal of the American Society for Mass Spectrometry · 2008Article
- Charge location directs electron capture dissociation of peptide dications.Journal of the American Society for Mass Spectrometry · 2006Article
- Fragmentation of oligoribonucleotides from gas-phase ion-electron reactions.Journal of the American Society for Mass Spectrometry · 2006Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electron capture dissociation (ECD) efficiency has typically been lower than for other dissociation techniques. Here we characterize experimental factors that limit ECD and seek to improve its efficiency. Efficiency of precursor to product ion conversion was measured for a range of peptide (approximately 15% efficiency) and protein (approximately 33% efficiency) ions of differing sizes and charge states. Conversion of precursor ions to products depends on electron irradiation period and maximizes at approximately 5-30 ms. The optimal irradiation period scales inversely with charge state. We demonstrate that reflection of electrons through the ICR cell is more efficient and robust than a single pass, because electrons can cool to the optimal energy for capture, which allows for a wide range of initial electron energy. Further, efficient ECD with reflected electrons requires only a short (approximately 500 micros) irradiation period followed by an appropriate delay for cooling and interaction. Reflection of the electron beam results in electrons trapped in or near the ICR cell and thus requires a brief (approximately 50 micros) purge for successful mass spectral acquisition. Further electron irradiation of refractory precursor ions did not result in further dissociation. Possibly the ion cloud and electron beam are misaligned radially, or the electron beam diameter may be smaller than that of the ion cloud such that remaining precursor ions do not overlap with the electron beam. Several ion manipulation techniques and use of a large, movable dispenser cathode reduce the possibility that misalignment of the ion and electron beams limits ECD efficiency.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.