ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
Temperature gradient capillary electrophoresis (TGCE)--a tool for the high-throughput discovery and mapping of SNPs and IDPs.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 35 citations in OpenAlex.
- Review
- Advances in ligase chain reaction and ligation-based amplifications for genotyping assays: Detection and applications.Mutation research. Reviews in mutation research · 2017Review
- EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations.Plant methods · 2014Article
- High-resolution genotyping via whole genome hybridizations to microarrays containing long oligonucleotide probes.PloS one · 2010Article
- High-throughput genetic mapping of mutants via quantitative single nucleotide polymorphism typing.Genetics · 2010Article
- Maize inbreds exhibit high levels of copy number variation (CNV) and presence/absence variation (PAV) in genome content.PLoS genetics · 2009Article
- Mutation detection using ENDO1: application to disease diagnostics in humans and TILLING and Eco-TILLING in plants.BMC molecular biology · 2008Article
- Role of RAD51 in the repair of MuDR-induced double-strand breaks in maize (Zea mays L.).Genetics · 2008Article
- Diversity in conserved genes in tomato.BMC genomics · 2007Article
- Unequal sister chromatid and homolog recombination at a tandem duplication of the A1 locus in maize.Genetics · 2006Article
- GRAMA: genetic mapping analysis of temperature gradient capillary electrophoresis data.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
Corrections and comments
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8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Temperature gradient capillary electrophoresis (TGCE) can be used to distinguish heteroduplex from homoduplex DNA molecules and can thus be applied to the detection of various types of DNA polymorphisms. Unlike most single nucleotide polymorphism (SNP) detection technologies, TGCE can be used even in the absence of prior knowledge of the sequences of the underlying polymorphisms. TGCE is both sensitive and reliable in detecting SNPs, small InDel (insertion/deletion) polymorphisms (IDPs) and simple sequence repeats, and using this technique it is possible to detect a single SNP in amplicons of over 800 bp and 1-bp IDPs in amplicons of approximately 500 bp. Genotyping data obtained via TGCE are consistent with data obtained via gel-based detection technologies. For genetic mapping experiments, TGCE has a number of advantages over alternative heteroduplex-detection technologies such as celery endonuclease (CELI) and denaturing high-performance liquid chromatography (dHPLC). Multiplexing can increase TGCE's throughput to 12 markers on 94 recombinant inbreds per day. Given its ability to efficiently and reliably detect a variety of subtle DNA polymorphisms that occur at high frequency in genes, TGCE shows great promise for discovering polymorphisms and conducting genetic mapping and genotyping experiments.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.