ArticleProceedings of the National Academy of Sciences of the United States of America2005
Rapid identification and strain-typing of respiratory pathogens for epidemic surveillance.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
84 citing papers in PubMed, 173 citations in OpenAlex.
- Rapid PCR/ESI-MS-based molecular genotyping of Staphylococcus aureus from nasal swabs of emergency department patients.BMC infectious diseases · 2014Trial
- A Comparison between Two Pathophysiologically Different yet Microbiologically Similar Lung Diseases: Cystic Fibrosis and Chronic Obstructive Pulmonary Disease.International journal of respiratory and pulmonary medicine · 2018Article
- Recent Advances in Molecular Diagnosis ofFrontiers in microbiology · 2018Article
- Broad-range survey of vector-borne pathogens and tick host identification of Ixodes ricinus from Southern Czech Republic.FEMS microbiology ecology · 2017Article
- DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan.BMC microbiology · 2016Article
- Article
- Identification of Microorganisms by High Resolution Tandem Mass Spectrometry with Accurate Statistical Significance.Journal of the American Society for Mass Spectrometry · 2016Article
- Simultaneous Extraction of Viral and Bacterial Nucleic Acids for Molecular Diagnostic Applications.Journal of biomolecular techniques : JBT · 2015Article
- Evaluation of a PCR/ESI-MS platform to identify respiratory viruses from nasopharyngeal aspirates.Journal of medical virology · 2015Article
- Sterile and microbial-associated intra-amniotic inflammation in preterm prelabor rupture of membranes.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015Article
- Sterile intra-amniotic inflammation in asymptomatic patients with a sonographic short cervix: prevalence and clinical significance.The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians · 2015Article
- A Case of Bordetella brochiseptica at a Military Medical Facility in Hawai'i: Phenotypic and Molecular Testing of an Uncommon Human Pathogen.Hawai'i journal of medicine & public health : a journal of Asia Pacific Medicine & Public Health · 2015Article
- Article
- Broad-range survey of tick-borne pathogens in Southern Germany reveals a high prevalence of Babesia microti and a diversity of other tick-borne pathogens.Vector borne and zoonotic diseases (Larchmont, N.Y.) · 2014Article
- Article
- A novel molecular microbiologic technique for the rapid diagnosis of microbial invasion of the amniotic cavity and intra-amniotic infection in preterm labor with intact membranes.American journal of reproductive immunology (New York, N.Y. : 1989) · 2014Article
- Diagnosis of prosthetic joint infection by use of PCR-electrospray ionization mass spectrometry.Journal of clinical microbiology · 2014Article
- Improving the diagnosis of bloodstream infections: PCR coupled with mass spectrometry.BioMed research international · 2014Review
- Review
- Evolving gene targets and technology in influenza detection.Molecular diagnosis & therapy · 2013Review
24 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
30 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epidemic respiratory infections are responsible for extensive morbidity and mortality within both military and civilian populations. We describe a high-throughput method to simultaneously identify and genotype species of bacteria from complex mixtures in respiratory samples. The process uses electrospray ionization mass spectrometry and base composition analysis of PCR amplification products from highly conserved genomic regions to identify and determine the relative quantity of pathogenic bacteria present in the sample. High-resolution genotyping of specific species is achieved by using additional primers targeted to highly variable regions of specific bacterial genomes. This method was used to examine samples taken from military recruits during respiratory disease outbreaks and for follow up surveillance at several military training facilities. Analysis of respiratory samples revealed high concentrations of pathogenic respiratory species, including Haemophilus influenzae, Neisseria meningitidis, and Streptococcus pyogenes. When S. pyogenes was identified in samples from the epidemic site, the identical genotype was found in almost all recruits. This analysis method will provide information fundamental to understanding the polymicrobial nature of explosive epidemics of respiratory disease.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.